A single‐step method for isolation of specific cells based on multiple surface markers will have unique advantages because of its scalability, efficacy, and mildness. Herein, we developed multi‐aptamer‐mediated proximity ligation method on live cell membranes that leverages a multi‐receptor co‐recognition design for enhanced specificity, as well as a robust in situ signal amplification design for improved sensitivity of cell isolation. We demonstrated the promising efficacy of our method on differentiating tumor cell subtypes in both cell mixtures and clinical samples. Owing to its simple and fast operation with excellent cell isolation sensitivity and accuracy, this approach will have broad applications in biological science, biomedical engineering, and personalized medicine.

中文翻译：

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高特异性，一步法分离癌细胞，对活细胞膜进行多适体介导的邻近结扎。

基于多种表面标记的单步分离特定细胞的方法将具有独特的优势，因为它具有可扩展性，功效和温和性。本文中，我们开发了一种在活细胞膜上的多适体介导的邻近连接方法，该方法利用多受体共识别设计来增强特异性，并使用强大的原位信号放大设计来提高细胞分离的敏感性。我们证明了我们的方法在区分细胞混合物和临床样品中肿瘤细胞亚型方面的有希望的功效。由于其简单，快速的操作以及出色的细胞分离灵敏度和准确性，该方法将在生物科学，生物医学工程和个性化医学中得到广泛应用。