Embryo implantation plays a decisive role in pregnancy. While in the process of implantation, microRNA (miRNA) is an important regulatory factor in the post transcriptional level. However, the role of many miRNAs in embryo implantation remained unknown. In this study, microRNA-183 (miR-183) was found differentially expressed in mouse uterus during implantation. In vivo treatment of miR-183 agomir in the uterine horn before implantation could eliminate the number of implantation site. The localization of miR-183 in mouse uteri gradually changed from epithelial to stromal layer in early pregnancy. Mice implantation models demonstrated that the decrease of miR-183 was mainly caused by maternal factors. Loss and gain function of miR-183 in endometrial cell lines showed that miR-183 could inhibit cell migration, invasion and apoptosis. MiR-183 could inhibit embryo implantation by binding Heparin-Binding EGF-like growth factor (Hbegf) and Laminin gamma one (Lamc1), which were key genes in embryo apposition and penetration. All these evidences indicate that miR-183 plays an important role during embryo implantation. This study provides new insights into the functions of miR-183 during embryo implantation and the development of contraceptive drugs in early pregnancy.

中文翻译：

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MiR-183通过调节小鼠子宫中的Hbegf和Lamc1来阻碍胚胎着床

胚胎植入在怀孕中起着决定性的作用。在植入过程中，微小RNA（miRNA）是转录后水平的重要调控因子。然而，许多 miRNA 在胚胎植入中的作用仍然未知。在这项研究中，发现 microRNA-183 (miR-183) 在植入过程中在小鼠子宫中差异表达。植入前子宫角中miR-183 agomir的体内处理可以消除植入部位的数量。miR-183在小鼠子宫中的定位在妊娠早期逐渐从上皮层转变为基质层。小鼠植入模型表明，miR-183的减少主要是由母体因素引起的。miR-183在子宫内膜细胞系中的丢失和获得功能表明，miR-183可以抑制细胞迁移、侵袭和凋亡。MiR-183 可以通过结合肝素结合 EGF 样生长因子 (Hbegf) 和层粘连蛋白伽玛一 (Lamc1) 来抑制胚胎植入，这些是胚胎并置和穿透的关键基因。所有这些证据表明 miR-183 在胚胎植入过程中起着重要作用。这项研究为 miR-183 在胚胎植入过程中的功能和妊娠早期避孕药物的开发提供了新的见解。