Abstract An electrochemical d -fructose sensor was fabricated by immobilizing a variant of d -fructose dehydrogenase on a porous gold microelectrode. The enzyme-modified electrode bioelectrocatalytically oxidizes d -fructose via direct electron transfer. The catalytic current reaches a steady-state limiting value at 0.05 V vs. Ag|AgCl (sat. KCl). The temperature dependence of the sensor suggests that its response is limited by the diffusion of d -fructose. Therefore, the sensor requires no calibration. The sensitivity of the sensor, (2.0 ± 0.2) × 102 μA cm−2 mM−1, is reproducible. The upper limit of detection is ~2.0 mM and the kinetics of the bioelectrocatalytic reaction is determined the limitation. The fabricated sensors were applied in the determination of d -fructose in commercial beverages as well as honey, and compared favorably with a more commonly used photometric method.

中文翻译：

---

基于 d-果糖脱氢酶变体在多孔金微电极上的直接电子转移型生物电催化的扩散限制电化学 d-果糖传感器

摘要 通过将d-果糖脱氢酶的变体固定在多孔金微电极上，制备了电化学d-果糖传感器。酶修饰电极生物电催化通过直接电子转移氧化 d-果糖。催化电流在 0.05 V vs. Ag|AgCl（饱和 KCl）时达到稳态极限值。传感器的温度依赖性表明其响应受到 d-果糖扩散的限制。因此，传感器不需要校准。传感器的灵敏度 (2.0 ± 0.2) × 102 μA cm-2 mM-1，是可重现的。检测上限为~2.0 mM，生物电催化反应的动力学决定了该限制。制造的传感器用于测定商业饮料和蜂蜜中的 d-果糖，