Protein, as the material basis of vita, is the crucial undertaker of life activities, which constitutes the framework and main substance of human tissues and organs, and takes part in various forms of life activities in organisms. Separating proteins from biomaterials and studying their structures and functions are of great significance for understanding the law of life activities and clarifying the essence of life phenomena. Therefore, scientists have proposed the new concept of proteomics, in which protein separation technology plays a momentous role. It has been diffusely used in the food industry, agricultural biological research, drug development, disease mechanism, plant stress mechanism, and marine environment research. In this paper, combined with the recent research situation, the progress of protein separation technology was reviewed from the aspects of extraction, precipitation, membrane separation, chromatography, electrophoresis, molecular imprinting, microfluidic chip and so on.

蛋白质是维他命的物质基础，是生命活动的重要承担者，它构成人体组织和器官的框架和主要物质，并参与生物体各种形式的生命活动。从生物材料中分离蛋白质并研究其结构和功能对于理解生命活动的规律和阐明生命现象的本质具有重要意义。因此，科学家提出了蛋白质组学的新概念，其中蛋白质分离技术起着举足轻重的作用。它已广泛用于食品工业，农业生物学研究，药物开发，疾病机理，植物胁迫机理和海洋环境研究。本文结合最近的研究现状，