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Tocopherol Moderately Induces the Expressions of Some Human Sulfotransferases, which are Activated by Oxidative Stress.
Cell Biochemistry and Biophysics ( IF 2.6 ) Pub Date : 2020-09-08 , DOI: 10.1007/s12013-020-00938-x
Sangita MaitiDutta 1 , Guangping Chen 2 , Smarajit Maiti 3, 4
Affiliation  

Oxidative stress is generated in biological system by several endogenous/exogenous factors like environmental-pollution/toxicity/diseases and by daily-life-stress. We previously showed that oxidative-stress impaired the activities/expressions of phase-II drug-metabolizing enzyme, sulfotransferases (SULTs). The SULT catalyzes sulfation of endogenous/exogenous compounds. Vitamin E is globally consumed by a large number of individuals for the cellular protection from oxidative stress and aging. Here, vitamin E (tocopherol; α/γ and tocotrienol; α/γ; 0, 1, 10, or 100 μM) was tested in human carcinoma cell line, HepG2 for their influences on SULTs expression/(western blotting). The effects of oxidant (glutathione-oxidized/GSSG) or reductant (glutathione-reduced/GSH, Dithiothreitol/DTT) on SULT activities were studied in rat-liver/human intestinal tissues. Results suggest, tocopherol is more inductive to monoamine-SULT (MPST) and Dehydroepiandrosterone-SULT (DHEAST) compared to that of tocotrienol (inconsistent change in PPST, phenol sulfotransferase/MPST/EST, estrogen sulfotransferase). The nuclear-factor constitutive androstane receptor (CAR) was found to be induced moderately. This study overall describes that vitamin E moderately influences SULTs expression. The induction ability of tocopherol should be judged taking into account its long-term consummation. Oxidative stress activates rat and human SULTs activities and expressions. Further studies are necessary in this regard.



中文翻译:

生育酚中度诱导一些由氧化应激激活的人类磺基转移酶的表达。

氧化应激是由环境污染/毒性/疾病等几种内源性/外源性因素和日常生活压力在生物系统中产生的。我们之前表明,氧化应激会损害 II 期药物代谢酶磺基转移酶 (SULT) 的活性/表达。SULT 催化内源性/外源性化合物的硫酸化。维生素 E 在全球范围内被大量个人食用,以保护细胞免受氧化应激和衰老。在这里,在人类癌细胞系 HepG2 中测试了维生素 E(生育酚;α/γ 和生育三烯酚;α/γ;0、1、10 或 100 μM)对 SULT 表达的影响/(蛋白质印迹)。氧化剂(glutathione-oxidized/GSSG)或还原剂(glutathione-reduced/GSH)的影响,在大鼠肝脏/人肠道组织中研究了二硫苏糖醇/DTT) 对 SULT 活性的影响。结果表明,与生育三烯酚相比,生育酚对单胺-SULT (MPST) 和脱氢表雄酮-SULT (DHEAST) 的诱导更大(PPST、苯酚磺基转移酶/MPST/EST、雌激素磺基转移酶的变化不一致)。发现核因子组成型雄甾烷受体(CAR)被适度诱导。这项研究总体上描述了维生素 E 适度影响 SULTs 表达。生育酚诱导能力的判断应考虑其长期完善。氧化应激激活大鼠和人类 SULTs 的活动和表达。在这方面需要进一步的研究。与生育三烯酚相比,生育酚对单胺-SULT (MPST) 和脱氢表雄酮-SULT (DHEAST) 的诱导性更强(PPST、苯酚磺基转移酶/MPST/EST、雌激素磺基转移酶的变化不一致)。发现核因子组成型雄甾烷受体(CAR)被适度诱导。这项研究总体上描述了维生素 E 适度影响 SULTs 表达。生育酚诱导能力的判断应考虑其长期完善。氧化应激激活大鼠和人类 SULTs 的活动和表达。在这方面需要进一步的研究。与生育三烯酚相比,生育酚对单胺-SULT (MPST) 和脱氢表雄酮-SULT (DHEAST) 的诱导性更强(PPST、苯酚磺基转移酶/MPST/EST、雌激素磺基转移酶的变化不一致)。发现核因子组成型雄甾烷受体(CAR)被适度诱导。这项研究总体上描述了维生素 E 适度影响 SULTs 表达。生育酚诱导能力的判断应考虑其长期完善。氧化应激激活大鼠和人类 SULTs 的活动和表达。在这方面需要进一步的研究。这项研究总体上描述了维生素 E 适度影响 SULTs 表达。生育酚诱导能力的判断应考虑其长期完善。氧化应激激活大鼠和人类 SULTs 的活动和表达。在这方面需要进一步的研究。这项研究总体上描述了维生素 E 适度影响 SULTs 表达。生育酚诱导能力的判断应考虑其长期完善。氧化应激激活大鼠和人类 SULTs 的活动和表达。在这方面需要进一步的研究。

更新日期:2020-09-08
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