Backgrounds

In this study, Jellyfish extract at immunity reaction (JEI) captured Ag citrate nanoparticles (Ag citrate NPs) and reduce toxicity was investigated.

Methods

JEI obtained from Aurelia aurita was used to assess the capture of Ag citrate NPs (10 mg/L, 2 mg/L) during 24 h, and the toxicity of JEI was assessed using a human cell line BEAS-2B.

Results

JEI was to reduce Ag citrate NPs in water by 34.55% (10 mg/L) after 1 h and by 40.62 ~ 41% (2 mg/L, 10 mg/L) after 24 h. When the BEAS-2B was exposed to 2 mg/L of Ag citrate NPs mixed with JEI showed increase 33.97% survival than 2 mg/L of Ag citrate NPs (0%). Furthermore, the apoptosis-related genes (p53, Bcl2, and Bax) showed no significant difference in expression upon exposure to JEI, compared to the control.

Conclusion

JEI is to capture Ag citrate NPs and reduce its nano-toxicity. Therefore, such ability of JEI is likely to prove useful in the control of AgNPs flowing into the aquatic ecosystem.

中文翻译：

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水母提取物对柠檬酸银纳米颗粒的捕获和毒性评估

背景资料

在这项研究中，对免疫反应（JEI）上的海ly提取物捕获了柠檬酸Ag纳米颗粒（Ag citrate NPs）并降低了毒性。

方法

从Aurelia aurita获得的JEI用于评估24小时内柠檬酸Ag NPs（10 mg / L，2 mg / L）的捕获，并使用人细胞系BEAS-2B评估JEI的毒性。

结果

JEI将在1小时后将水中的柠檬酸银NP减少34.55％（10 mg / L），并在24小时后将其减少40.62〜41％（2 mg / L，10 mg / L）。当BEAS-2B暴露于2 mg / L柠檬酸Ag NPs与JEI混合时，其存活率比2 mg / L柠檬酸Ag NPs（0％）增加了33.97％。此外，与对照相比，凋亡相关基因（p53，Bcl2和Bax）在暴露于JEI后表达无显着差异。

结论

JEI将捕获柠檬酸银NP，并降低其纳米毒性。因此，JEI的这种能力可能被证明可用于控制流入水生生态系统的AgNP。