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An analysis of exo-polygalacturonase bioprocess in submerged and solid-state fermentation by Pleurotus ostreatus using pomelo peel powder as carbon source.
Journal of Genetic Engineering and Biotechnology Pub Date : 2020-09-07 , DOI: 10.1186/s43141-020-00061-7
Kausik Majumder 1, 2 , Bubai Paul 1 , Rakhi Sundas 1
Affiliation  

As there has been an increasing trend in the effective utilization of plant and crop residues for microbial transformation into a desired product, an attempt was made to compare of exo-polygalacturonase production using logistic and Luedeking-Piret kinetic model by Pleurotus ostreatus in submerged (smf) or solid-state fermentation (ssf) using pomelo peel powder, an agro-forestry residue as carbon substrate. Cultures grown in submerged fermentation produced a peak of exo-polygalacturonase activity as 6160 Ul-1 on the 4th day of culture as compared with 2410 Ul-1 on the 5th day of fermentation by solid-state fermentation. The enzyme yield coefficient (YE/X) is of higher value in smf vs. ssf system (YE/X = 1.05 × 103 vs. 0.622 × 103) indicating the more efficient product yield in smf as compared with ssf. The plots derived fromλ versusζ clearly demonstrate that the secondary product destruction is higher in smf than in ssf. P. ostreatus performs much better in submerged fermentation as compared with solid-state fermentation in respect to exo-polygalacturonase production although ssf technique produced a more thermo-stable exo-polygalacturonase in crude extract, which is highly desirable in various industrial applications.

中文翻译:

以柚皮粉为碳源的平菇发酵和固态发酵过程中外半乳糖苷酶的生物过程分析。

由于有效利用植物和农作物残留物将微生物转化为所需产品的趋势有所增加,因此尝试通过平菇和平菇在深水(smf)中使用logistic和Luedeking-Piret动力学模型比较外泌多聚半乳糖醛酸酶的生产。 )或使用柚子果皮粉(一种农林业残留物作为碳底物)进行固态发酵(ssf)。通过固态发酵,在水下发酵中生长的培养物在培养的第4天产生的外切-聚半乳糖醛酸酶活性峰值为6160 Ul-1,而在发酵的第5天则为2410 Ul-1。在smf与ssf系统中,酶产率系数(YE / X)值更高(YE / X = 1.05×103与0.622×103),表明在smf中产品产量比ssf更有效。从λ对ζ得出的图清楚地表明,smf中的二次产物破坏高于ssf中的二次产物破坏。在固态发酵中,相比于固态发酵,平菇在外发酵多聚半乳糖醛酸酶生产方面要好得多,尽管自卫队技术在粗提取物中产生了更热稳定的外聚半乳糖醛酸酶,这在各种工业应用中都是非常需要的。
更新日期:2020-09-08
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