ABSTRACT

Setaria italica, Panicum miliaceum

var. compactum, and Chenopodium quinoa have strong stress resistance, allowing adaptation to poor environments. The study of the stress resistance mechanisms of these crops has important significance for humans. The microbial diversity and metabolic pathways in soil collected from the rhizospheres of S. italica, P.miliaceum var. compactum, and C.quinoa were investigated using high-throughput sequencing. The taxonomic composition analysis of the flora indicates that during seedling and flowering stages, compared with immature soil samples, the main differential genera were Brachybacterium (p < 0.05), Pimelobacter (p < 0.05) and Candidatus Nitrososphaera (p < 0.05) for C. quinoa rhizosphere soil samples; Glycomyces for S. italica rhizosphere soil samples (p < 0.05); and Pseudomonas for P.miliaceum var. compactum rhizosphere soil samples (p < 0.05). Functional prediction analysis showed that, in two different samples, the calcium signaling pathway in S. italica soil microbes was enriched. For P.miliaceum var. compactum and C. quinoa, the enriched metabolic pathways were clavulanic acid biosynthesis and biosynthesis of the type II polyketide backbone, respectively (all, p < 0.05). This study indicates that crop species and sampling times have significant effects on the diversity of rhizosphere microorganisms under immature soil conditions.

摘要

狗尾草，Panicum miliaceum

变种。compactum和Chenopodium quinoa具有很强的抗逆性，可以适应恶劣的环境。研究这些作物的抗逆机制对人类具有重要意义。从S. italica, P. miliaceum var.根际收集的土壤中的微生物多样性和代谢途径。使用高通量测序研究了compactum和C.quinoa。植物区系的分类组成分析表明，在苗期和开花期，与未成熟土壤样品相比，主要差异属为短杆菌属（p < 0.05）、Pimelobacter（p< 0.05) 和Candidatus Nitrososphaera ( p < 0.05) 用于C. quinoa根际土壤样品；Glycomyces为S.谷子根际土壤样品（p <0.05）; 和假单胞菌为P.miliaceum var。致密根际土壤样品（p <0.05）。功能预测分析表明，在两个不同的样品中，S. italica土壤微生物中的钙信号通路得到了丰富。对于P.miliaceum var。紧凑型和藜麦, 富集的代谢途径分别是克拉维酸的生物合成和 II 型聚酮化合物骨架的生物合成 (所有, p < 0.05)。本研究表明，在未成熟土壤条件下，作物种类和采样时间对根际微生物的多样性有显着影响。