ABSTRACT Cellular aging occurs as a cell loses its ability to maintain homeostasis. Aging cells eliminate damaged cellular compartments and other senescence factors via self-renewal. The mechanism that regulates cellular rejuvenation remains to be further elucidated. Using budding yeast gametogenesis as a model, we show here that the endosomal sorting complex required for transport (ESCRT) III regulates nuclear envelope organization. During gametogenesis, the nuclear pore complex (NPC) and other senescence factors are sequestered away from the prospore nuclei. We show that the LEM-domain protein Heh1 (Src1) facilitates the nuclear recruitment of ESCRT-III, which is required for meiotic NPC sequestration and nuclear envelope remodeling. Furthermore, ESCRT-III-mediated nuclear reorganization appears to be critical for gamete rejuvenation, as hindering this process curtails either directly or indirectly the replicative lifespan in gametes. Our findings demonstrate the importance of ESCRT-III in nuclear envelope remodeling and its potential role in eliminating senescence factors during gametogenesis.

中文翻译：

---

ESCRT-III 复合物是核孔复合物隔离所必需的，并调节芽殖酵母减数分裂中的配子复制寿命

摘要 当细胞失去维持体内平衡的能力时，就会发生细胞老化。衰老细胞通过自我更新消除受损的细胞区室和其他衰老因素。调节细胞再生的机制仍有待进一步阐明。使用芽殖酵母配子发生作为模型，我们在这里展示了运输所需的内体分选复合物 (ESCRT) III 调节核膜组织。在配子发生过程中，核孔复合物 (NPC) 和其他衰老因子与孢子核隔离。我们显示 LEM 域蛋白 Heh1 (Src1) 促进 ESCRT-III 的核募集，这是减数分裂 NPC 隔离和核膜重塑所必需的。此外，ESCRT-III 介导的核重组似乎对配子再生至关重要，因为阻碍这一过程会直接或间接地缩短配子的复制寿命。我们的研究结果证明了 ESCRT-III 在核膜重塑中的重要性及其在消除配子发生过程中衰老因子方面的潜在作用。