In this study, the performance of biosurfactants produced by Enterobacter cloacae (PTCC:1798) and Acintobacter calcoaceticus (PTCC:1318) is investigated and the optimum values of operational parameters such as salinity%, MEOR type, and microorganism type to enhance the recovery from oil reservoirs are introduced. A comparison between ex-situ and in-situ injection in micromodel is also provided and the efficiency of produced biosurfactants in EOR is investigated for the first time in the literature. Experiments were designed using general factorial method in Design Expert software. In order to do that, design of experiment (DOE) followed by experimental flooding tests into a micromodel and response surface methodology (RSM) were employed. According to experimental design, four levels of salt concentration (0, 3%, 6%, and 12%), two levels of injection type (in-situ and ex-situ), and two levels of bacteria species were examined. The experiment results indicate a maximum oil recovery factor of 58% and 54% for in-situ injection obtained by Acintobacter at 3% salinity and Enterobacter at 6% salinity, respectively. The optimum oil recovery factors of 67% and 59% are achieved by ex-situ injection of water-biosurfactant from Acintobacter in CMC condition and 3% salinity and Enterobacter in CMC condition and 6% salinity, respectively.

在这项研究中，阴沟肠杆菌（PTCC：1798）和钙不动杆菌生产的生物表面活性剂的性能（PTCC：1318）进行了研究，并引入了诸如盐度％，MEOR类型和微生物类型等操作参数的最佳值，以提高从油藏中的采收率。还提供了微模型中异位注射和原位注射之间的比较，并且在文献中首次研究了在EOR中生产的生物表面活性剂的效率。实验是在Design Expert软件中使用一般析因法设计的。为了做到这一点，采用了实验设计（DOE），接着进行了将实验洪水测试转化为微模型和响应面方法（RSM）。根据实验设计，对盐浓度的四个级别（0％，3％，6％和12％），注射类型的两个级别（原位和异位）和细菌种类的两个级别进行了检查。盐度为3％的不动杆菌和盐度为6％的肠杆菌。通过在CMC条件下从不动杆菌中异位注入水生物表面活性剂，在CMC条件下分别从3％的盐度和肠杆菌中获得最佳的油采收率，分别为3％盐度和6％盐度。