Due to the rapid development of industrial society, air pollution is becoming a serious problem which has being a huge threat to human health. Ultrafine particles (UFPs), one of the major air pollutants, are often the culprits of human diseases. At present, most of the toxicological studies of UFPs focus on their biological effects on lung cells and tissues, but there are less researches taking aim at the negative effects on functional proteins within the body. Therefore, we experimentally explored the effects of ultrafine carbon black (UFCB) on the structure and function of trypsin. After a short‐term exposure to UFCB, the trypsin aromatic amino acid microenvironment, protein backbone and secondary structure were changed significantly, and the enzyme activity showed a trend that rose at first, then dropped. In addition, UFCB interacts with trypsin in the form of a complex. These studies demonstrated the negative effects of UFCB on trypsin, evidencing potential effects on animals and humans.

中文翻译：

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超细炭黑对胰蛋白酶结构和功能影响的研究。

随着工业社会的快速发展，空气污染日益严重，对人类健康构成巨大威胁。超细颗粒 (UFP) 是主要的空气污染物之一，通常是人类疾病的罪魁祸首。目前，UFPs的毒理学研究大多集中在其对肺细胞和组织的生物学作用上，而针对其对体内功能蛋白的负面作用的研究较少。因此，我们通过实验探索了超细炭黑（UFCB）对胰蛋白酶结构和功能的影响。UFCB短期暴露后，胰蛋白酶芳香氨基酸微环境、蛋白质骨架和二级结构发生明显变化，酶活性呈先上升后下降趋势。此外，UFCB 以复合物的形式与胰蛋白酶相互作用。这些研究证明了 UFCB 对胰蛋白酶的负面影响，证明了对动物和人类的潜在影响。