Abstract The growing uses of functional polymers in different domains and the well-established remanence of synthetic polymers underline the necessity to study their microbial biodegradation. A phylloplane bacterial collection was screened for its potential to overcome documented factors limiting polymer biodegradation, i.e. low cleavable long chain linear polymers and low bioavailable hydrophobic compounds. Acrylate-based polymers, namely poly(2-hydroxyethyl methacrylate) (pHEMA), poly(N-isopropylacrylamide) (pNIPAM), poly(acrylamide) (pAM) and poly(acrylic acid sodium salt) (pAS), and a hydrophobic crosslinker Epoxidized Soybean Oil Acrylate (ESOA) were used as selective substrates. OD monitoring of growth with these molecules as sole carbon sources were correlated with substrates alterations determined by 1H NMR, GPC and FTIR. Selected strains belong to Bacillales Family XII. Incertae Sedis, Bacillaceae, Nocardiaceae, Micrococcaceae, Enterobacteriaceae and Pseudomonadaceae. Bacillus species mainly developed with ESOA. Exiguobacterium sibiricum strains could grow with pHEMA and pNIPAM leading to polymer molecular weight decreases. Arthrobacter koreensis showed the highest alteration capacities of pHEMA with molecular weight decreases from 22,000 to 3,148 g/mol and the production of presumed aromatics and carbon chains.

中文翻译：

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从作物中分离的叶面细菌对功能聚合物的改变

摘要 功能性聚合物在不同领域的应用越来越多，合成聚合物的残留性已得到证实，这凸显了研究其微生物生物降解的必要性。筛选叶面细菌集合的潜力，以克服限制聚合物生物降解的已记录因素，即低可裂解长链线性聚合物和低生物利用度疏水性化合物。基于丙烯酸酯的聚合物，即聚（2-羟乙基甲基丙烯酸酯）（pHEMA）、聚（N-异丙基丙烯酰胺）（pNIPAM）、聚（丙烯酰胺）（pAM）和聚（丙烯酸钠盐）（pAS），以及疏水性交联剂环氧大豆油丙烯酸酯 (ESOA) 用作选择性底物。以这些分子作为唯一碳源的生长的 OD 监测与由 1H NMR、GPC 和 FTIR 确定的底物变化相关。所选菌株属于芽孢杆菌属 XII。Incertae Sedis、芽孢杆菌科、诺卡氏菌科、微球菌科、肠杆菌科和假单胞菌科。芽孢杆菌属主要与 ESOA 一起发展。西伯利亚微小杆菌菌株可以与 pHEMA 和 pNIPAM 一起生长，导致聚合物分子量降低。朝鲜节杆菌表现出最高的 pHEMA 改变能力，分子量从 22,000 降至 3,148 g/mol，并产生假定的芳烃和碳链。