Single-cell RNA sequencing has revolutionized ocular gene expression studies. This technology has enabled researchers to identify expression signatures for rare cell types and characterize how gene expression changes across biological conditions, such as topographic region or disease status. However, sharing single-cell RNA sequencing results remains a major obstacle, particular for individuals without a computational background. To address these limitations, we developed Spectacle, an interactive web-based resource for exploring previously published single-cell RNA sequencing data from ocular studies. Spectacle is powered by a locally developed R package, cellcuratoR, which utilizes the Shiny framework in R to generate interactive visualizations for single-cell expression data. Spectacle contains five pre-processed ocular single-cell RNA sequencing data sets and is accessible via the web at OcularGeneExpression.org/singlecell. With Spectacle, users can interactively identify which cell types express a gene of interest, detect transcriptomic subpopulations within a cell type, and perform highly flexible differential expression analyses. The freely-available Spectacle system reduces the bioinformatic barrier for interacting with rich single-cell RNA sequencing studies from ocular tissues, making it easy to quickly identify cell types that express a gene of interest.

单细胞 RNA 测序彻底改变了眼部基因表达研究。这项技术使研究人员能够识别稀有细胞类型的表达特征，并表征基因表达如何在生物条件下发生变化，例如地形区域或疾病状态。然而，共享单细胞 RNA 测序结果仍然是一个主要障碍，特别是对于没有计算背景的个人。为了解决这些限制，我们开发了 Spectacle，这是一种基于网络的交互式资源，用于探索先前发布的来自眼部研究的单细胞 RNA 测序数据。Spectacle 由本地开发的 R 包 cellcuratoR 提供支持，它利用 R 中的 Shiny 框架为单细胞表达数据生成交互式可视化。Spectacle 包含五个预处理的眼部单细胞 RNA 测序数据集，可通过网络访问 OcularGeneExpression.org/singlecell。使用 Spectacle，用户可以交互识别哪些细胞类型表达感兴趣的基因，检测细胞类型内的转录组亚群，并执行高度灵活的差异表达分析。免费提供的 Spectacle 系统减少了与来自眼组织的丰富单细胞 RNA 测序研究相互作用的生物信息学障碍，从而可以轻松快速识别表达感兴趣基因的细胞类型。并进行高度灵活的差异表达分析。免费提供的 Spectacle 系统减少了与来自眼组织的丰富单细胞 RNA 测序研究相互作用的生物信息学障碍，从而可以轻松快速地识别表达感兴趣基因的细胞类型。并进行高度灵活的差异表达分析。免费提供的 Spectacle 系统减少了与来自眼组织的丰富单细胞 RNA 测序研究相互作用的生物信息学障碍，从而可以轻松快速地识别表达感兴趣基因的细胞类型。