Abstract Background Cellulose as a potential feed resource hinders its utilization because of its complex structure, and cellulase is the key to its biological effective utilization. Animal endogenous probiotics are more susceptible to colonization in the intestinal tract, and their digestive enzymes are more conducive to the digestion and absorption of feed in young animals. Min pigs are potential sources of cellulase probiotics because of the high proportion of dietary fiber in their feed. In this study, the cellulolytic bacteria in the feces of Min pigs were isolated and screened. The characteristics of enzymes and cellulase production were studied, which provided a theoretical basis for the rational utilization of cellulase and high-fiber food in animal production. Results In our study, 10 strains of cellulase producing strains were isolated from Min pig manure, among which the M2 strain had the best enzyme producing ability and was identified as Bacillus velezensis. The optimum production conditions of cellulase from strain M2 were: 2% inoculum, the temperature of 35°C, the pH of 5.0, and the liquid loading volume of 50 mL. The optimum temperature, pH and time for the reaction of cellulase produced by strain M2 were 55°C, 4.5 and 5 min, respectively. Conclusions Min pigs can be used as a source of cellulase producing strains. The M2 strain isolated from feces was identified as Bacillus velezensis. The cellulase from M2 strain had a good activity and the potential to be used as feed additive for piglets. How to cite: Li F, Xie Y, Gao X, et al. Screening of cellulose degradation bacteria from Min Pigs and optimization of its cellulase production. Electron J Biotechnol 2020;48. https://doi.org/10.1016/j.ejbt.2020.09.001

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小猪纤维素降解菌的筛选及纤维素酶生产优化

摘要 背景纤维素作为一种潜在的饲料资源，其结构复杂，阻碍了其利用，而纤维素酶是其生物有效利用的关键。动物内源性益生菌更容易在肠道定植，其消化酶更有利于幼畜饲料的消化吸收。小猪是纤维素酶益生菌的潜在来源，因为它们饲料中膳食纤维的比例很高。本研究对闽猪粪便中的纤维素分解菌进行了分离筛选。研究了酶和纤维素酶生产的特性，为纤维素酶和高纤维食品在动物生产中的合理利用提供了理论依据。结果 在我们的研究中，从闽猪粪中分离出10株产纤维素酶菌株，其中M2菌株产酶能力最好，经鉴定为Bacillus velezensis。菌株M2纤维素酶的最佳生产条件为：接种量2%，温度35℃，pH 5.0，加液量50 mL。菌株M2产生的纤维素酶反应的最适温度、pH值和反应时间分别为55℃、4.5和5分钟。结论闽猪可作为纤维素酶生产菌株的来源。从粪便中分离出的 M2 菌株被鉴定为 Bacillus velezensis。M2菌株的纤维素酶具有良好的活性，具有作为仔猪饲料添加剂的潜力。如何引用：Li F, Xie Y, Gao X, et al. 闽猪纤维素降解菌的筛选及纤维素酶生产优化[J]. 电子 J 生物技术 2020；48。https://doi.org/10.1016/j.ejbt.2020.09.001