Abstract Background Meretrix petechialis is one of the commercially important marine bivalves. In this study, we selected six highly polymorphic EST-derived microsatellite markers to assess the genetic diversity and population differentiation on nine wild populations of Meretrix petechialis. Results The number of alleles detected per locus ranged from 4 to 30 (mean NA = 27.5) with a total of 165 alleles. The mean value of observed and expected heterozygosities varied from 0.717 to 0.861 and from 0.797 to 0.856, respectively. Meanwhile, the result of Neighbor-joining and overall FST = 0.214 (P Conclusions GX population has high genetic diversity among individual, and there are certain differences in genetic characteristics among different populations. This study will provide a basis for the domestication and cultivation of genetic diversity of M. petechialis population and the protection of clam germplasm resources. How to cite Xu Q, Zheng J, Yan X, et al. Genetic diversity and differentiation of nine populations of the hard clam (Meretrix petechialis) assessed by EST-derived microsatellites. Electron J Biotechnol 2020;48. https://doi.org/10.1016/j.ejbt.2020.09.003

中文翻译：

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通过 EST 微卫星评估的九个硬蛤 (Meretrix petechialis) 种群的遗传多样性和分化

摘要 背景 Meretrix petechialis 是具有重要商业价值的海洋双壳类动物之一。在这项研究中，我们选择了六个高度多态性的 EST 衍生微卫星标记来评估 Meretrix petechialis 九个野生种群的遗传多样性和种群分化。结果 每个基因座检测到的等位基因数量从 4 到 30（平均 NA = 27.5）不等，总共有 165 个等位基因。观察到的和预期的杂合度的平均值分别从 0.717 到 0.861 和从 0.797 到 0.856 不等。同时，Neighbor-joining和整体FST=0.214（P结论GX种群个体间遗传多样性高，不同种群间遗传特征存在一定差异。本研究将为遗传的驯化和培育提供依据。 M的多样性 蛤蜊种群与蛤种质资源保护. 如何引用徐Q，郑杰，严X，等。通过 EST 衍生的微卫星评估九个硬蛤（Meretrix petechialis）种群的遗传多样性和分化。电子 J 生物技术 2020；48。https://doi.org/10.1016/j.ejbt.2020.09.003