Abstract Downy blight caused by Peronophythora litchii is one of the most destructive diseases of lychee (Litchi chinensis Sonn.). Early diagnosis of the P. litchii pathogen should be the top priority for addressing disease epidemics and management. In this study, loop-mediated isothermal amplification (LAMP) assays targeting the M90 gene sequences were developed for specific detection of P. litchii. The detection limit of LAMP assay was 10 pg genomic DNA of P. litchii in 25 μL reaction mixtures. LAMP assay and nested polymerase chain reaction (PCR) assay showed higher sensitivity than conventional PCR. The LAMP assay could also detect the presence of P. litchii in infected leaves, fruits and soils. This study provides a simple, highly sensitive, rapid and reliable protocol for the early diagnosis of P. litchii on lychee.

中文翻译：

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环介导等温扩增法检测荔枝上的荔枝霜霉病菌

摘要 荔枝霜霉病是荔枝(Litchi chinensis Sonn.)最具破坏性的病害之一。荔枝假单胞菌病原体的早期诊断应该是解决疾病流行和管理的首要任务。在这项研究中，开发了针对 M90 基因序列的环介导等温扩增 (LAMP) 检测，用于特异性检测荔枝假单胞菌。LAMP 检测的检测限为 10 pg 荔枝假单胞菌基因组 DNA 在 25 μL 反应混合物中。LAMP 检测和巢式聚合酶链反应 (PCR) 检测显示出比传统 PCR 更高的灵敏度。LAMP 检测还可以检测受感染的叶子、果实和土壤中荔枝假单胞菌的存在。本研究为荔枝上荔枝假单胞菌的早期诊断提供了一种简单、高灵敏度、快速和可靠的方案。