Abstract Revealing the spatial and temporal distribution of bacteria in organic tea plantations is of great importance to clarify soil- and/or tea plant-associated community. In this study samples were collected from three rhizo-compartments of tea plant during four seasons, which were further subjected to community level physiological profiling and 16S rDNA next generation sequencing. Organic fertilization caused a short-term disturbance on pH, EC, and organic matter content of soil. Three rhizo-compartments harbored distinct bacterial community as revealed by PCA, PCoA and UPGMA analysis. Utilization of six carbon source categories was positively correlated with root surface community while negatively correlated with root interior community. Although there were variations in microbial parameters during four seasons, higher metabolic potential and diversity were observed in soil-associated rhizo-compartments. Bacterial families Gemmataceae, Pedosphaeraceae, and Solibacteraceae were more abundant in soil and root surface, while root interior were dominated by families Acidothermaceae, Burkholderiaceae, Ktedonobacteraceae, and Xanthobacteraceae. It is proposed that Burkholderiaceae and Xanthobacteraceae which dominate in root surface or root interior samples and other poorly studied phylogenetic groups may serve as potential candidates to study their interaction with tea plant.

中文翻译：

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有机茶园细菌代谢潜能及群落结构

摘要 揭示有机茶园细菌的时空分布对于阐明土壤和/或茶树相关群落具有重要意义。在本研究中，从四个季节的茶树的三个根室中收集样品，进一步进行群落水平生理分析和 16S rDNA 下一代测序。有机施肥对土壤的 pH、EC 和有机质含量造成短期干扰。PCA、PCoA 和 UPGMA 分析表明，三个根隔室具有不同的细菌群落。六种碳源类别的利用与根表面群落正相关，而与根内部群落负相关。尽管四个季节的微生物参数存在变化，在与土壤相关的根际间室中观察到更高的代谢潜力和多样性。土壤和根表面的细菌科Gemmataceae、Pedosphaeraceae 和Solibacteraceae 更为丰富，而根内部则以Acidothermaceae、Burkholderiaceae、Ktedonobacteraceae 和Xanthobacteraceae 科为主。提出在根表面或根内部样品中占主导地位的伯克霍尔德菌科和黄杆菌科以及其他研究不足的系统发育组可能作为研究它们与茶树相互作用的潜在候选者。和黄杆菌科。提出在根表面或根内部样品中占主导地位的伯克霍尔德菌科和黄杆菌科以及其他研究不足的系统发育组可能作为研究它们与茶树相互作用的潜在候选者。和黄杆菌科。提出在根表面或根内部样品中占主导地位的伯克霍尔德菌科和黄杆菌科以及其他研究不足的系统发育组可能作为研究它们与茶树相互作用的潜在候选者。