Tomato is a key vegetable crop cultivated in nearly all agricultural regions of the world. It is a protective food very rich in vitamin A and C. Worldwide two major biotic stresses namely root-knot nematode (Meloidogyne incognita) and tomato leaf curl virus (ToLCV) pose a serious threat to its production. Serious environmental concerns associated with the chemical pesticides used for management of these pests compel researchers to look for alternate management strategies. Many recent publications indicate engineering resistance through host delivered RNAi to be an important, sustainable and efficient tool for nematode and virus management. In this study, we have attempted to engineer resistance against M. incognita and ToLCV by stacking dsRNA constructs of the Integrase gene of M. incognita and AC4 genes of ToLCV, using co-transformation protocol in tomato. Transgenic events, so produced, were confirmed at the molecular level by PCR and southern blot. Such T₂ events showed absolute absence of leaf curl virus disease symptoms and 62–63% reduction in the number of galls, 51–70% reduction in the number of eggs, 31–38% reduction in number of eggs per egg mass and 66–81% reduction in nematode multiplication factor when compared to the untransformed control plants.The study provides an evidence for generating resistance through RNAi against multiple biotic stresses.

番茄是世界上几乎所有农业地区种植的重要蔬菜作物。它是一种富含维生素A和C的保护性食品。在世界范围内，两种主要的生物胁迫，即根结线虫（Meloidogyne incognita）和番茄卷叶病毒（ToLCV）对其生产构成了严重威胁。与用于控制这些害虫的化学农药相关的严重环境问题迫使研究人员寻找替代的管理策略。最近的许多出版物表明，通过宿主传递的RNAi产生的工程抗性是线虫和病毒管理的重要，可持续和有效的工具。在这项研究中，我们试图对工程师抵抗南方根结线虫和ToLCV通过堆叠的dsRNA构建整合的基因南方根结线虫和AC4 ToLCV的基因，使用共转化方案中番茄。如此产生的转基因事件通过PCR和Southern印迹在分子水平上得到证实。此类T ₂事件显示绝对没有叶卷曲病毒病症状，胆汁数量减少62-63％，鸡蛋数量减少51-70％，每鸡蛋质量的鸡蛋数量减少31-38％，66与未转化的对照植物相比，线虫繁殖因子降低了–81％。该研究提供了通过RNAi对多种生物胁迫产生抗性的证据。