Lanthipeptides are a subgroup of ribosomally encoded and post-translationally modified peptides (RiPPs) which frequently possess potent biological activity. Here we provide the first comprehensive bioinformatic analysis of the lanthipeptide-producing capability of the Salinispora genus, a marine actinomycete. One hundred twenty-two Salinispora arenicola, tropica, and pacifica genomic sequences were analyzed for lanthipeptide gene clusters, and the resulting 182 clusters were divided into seven groups based on sequence similarities. Group boundaries were defined based on LanB and LanM sequences with greater than 80% similarity within groups. Of the seven groups, six are predicted to encode class I lanthipeptides while only one group is predicted to encode class II lanthipeptides. Leader and core peptides were predicted for each cluster along with the number of possible lanthionine bridges. Notably, all of the predicted products of these clusters would represent novel lanthipeptide scaffolds. Of the 122 Salinispora genomes analyzed in this study, 92% contained at least one lanthipeptide gene cluster suggesting that Salinispora is a rich, yet untapped, source of lanthipeptides.

多肽是核糖体编码的和翻译后修饰的肽（RiPPs）的一个子集，它们通常具有有效的生物学活性。在这里，我们提供了对海洋放线菌Salinispora属的瘦肽产生能力的首次综合生物信息学分析。一百二十二条沙里氏菌，热带和太平洋分析基因组序列中的小肽基因簇，并根据序列相似性将所得的182个簇分为七个组。基于LanB和LanM序列定义组边界，组内相似度大于80％。在这七个组中，预计有六个编码I类多肽肽，而只有一组被编码为II类多肽肽。预测每个簇的前导肽和核心肽，以及可能的羊毛硫氨酸桥的数量。值得注意的是，这些簇的所有预测产物将代表新型的脂肽支架。在这项研究中分析的122个Salinispora基因组中，有92％包含至少一个长肽基因簇，这表明Salinispora 是丰富而尚未开发的瘦肽来源。