Abstract

Human colorectal adenocarcinoma cell line Caco-2 is often used as a model of healthy intestinal epithelium, in particular, in miRNA studies. The work of the enzymes Drosha and Dicer is an integral part of the process of miRNA formation. Inaccuracies in the work of these enzymes lead to a change in the nucleotide sequences of miRNAs with the formation of new isoforms, which, in turn, can change intracellular regulatory mechanisms. In the framework of this study, it was shown that the quantitative estimates of inaccuracies in Drosha and Dicer activity significantly differ between the specimens of normal colon tissue and malignant colorectal tumors.

中文翻译：

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大肠癌和正常结肠组织样本中Drosha和Dicer卵裂曲线的差异。

摘要

人结肠直肠腺癌细胞系Caco-2通常被用作健康肠上皮的模型，尤其是在miRNA研究中。Drosha和Dicer酶的工作是miRNA形成过程不可或缺的一部分。这些酶的工作不准确会导致miRNA核苷酸序列发生变化，并形成新的同工型，进而改变细胞内调控机制。在这项研究的框架中，表明正常结肠组织和恶性大肠肿瘤标本中Drosha和Dicer活性不准确的定量估计值显着不同。