Abstract

One of the main complications of pregnancy and causes of maternal and perinatal mortality is preeclampsia. The pathophysiology of preeclampsia is associated with the development of placenta and fetal hypoxia and secretion of a number of effective molecules. The human choriocarcinoma cell line BeWo b30 is often used as a model of the placental barrier. It was shown that oxyquinoline derivatives can mimic hypoxia by suppressing HIF-prolyl hydroxylases and the accumulation of HIF-1α. This effect also leads to a change in the expression of microRNAs and their target genes. However, with hypoxia in cells, not only the level of individual miRNAs but also the ratio of miRNA isoforms (isomiRs) can change, presumably due to inaccuracies in the work of the Drosha and Dicer enzymes. In this work, we showed a change in the expression of the factors involved in the maturation of miRNAs when simulating hypoxia in BeWo b30 cells with an oxyquinoline derivative, which may be one of the causes for the change in the ratio of miRNA isoforms.

中文翻译：

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模仿miRNA加工的因素改变了BeWo b30细胞在缺氧过程中的表达水平。

摘要

子痫前期是怀孕的主要并发症之一，也是造成孕产妇和围产期死亡的原因之一。先兆子痫的病理生理与胎盘和胎儿缺氧的发展以及许多有效分子的分泌有关。人类绒癌组织细胞系BeWo b30通常被用作胎盘屏障的模型。结果表明，氧喹啉衍生物可以通过抑制HIF-脯氨酰羟化酶和HIF-1α的积累来模拟缺氧。这种作用还导致microRNA及其靶基因的表达发生变化。但是，由于细胞缺氧，不仅是单个miRNA的水平，而且miRNA亚型（isomiRs）的比率也会发生变化，这可能是由于Drosha和Dicer酶的工作不准确所致。在这项工作中