The design and synthesis of red light-emitting multianalyte chemosensors have always been a challenging task because of its specific requirement of coordination pocket and selective fluorescence mechanism. Herein, we develop a chemosensor via “three birds, one stone” strategy in which we can detect three metal ions with one ligand. A highly sensitive new azo functionalized rhodamine based luminescent sensor is synthesized for selective fluorogenic recognition of Al³⁺, Cr³⁺, and chromogenic recognition of Cu²⁺ in ethanol : H₂O medium in the red light-emitting zone. Among the guest metals, Cu²⁺ efficiently quenches the emission whereas Al³⁺ and Cr³⁺ induce increased luminescent 4.76 fold for Al³⁺ and 2.47 fold for Cr³⁺ through chelation-enhanced fluorescence (CHEF) and photo-induced electron transfer (PET) regulated mechanism with the formation of 1:1 complex. The restricted imine isomerization through complex formation inhibits ongoing PET process with the instantaneous onset of CHEF. The mechanism is in good consonance with NMR (¹H & ¹³C), FT-IR, elemental analysis, DFT, TCSPC, and pH-dependent studies. Micromolar range detection of 1.1 µM, 1.3 µM, and 1.5 µM for Cu²⁺, Al³⁺, and Cr³⁺ respectively, easy penetration into HLCs cells and higher imaging resolution increase its potentiality to assess Al³⁺ and Cr³⁺ in vitro. Moreover, paper strip application increases its viability as an onsite naked-eye portable solid probe.

红色发光的多分析物化学传感器的设计和合成一直是一项艰巨的任务，因为它对配位袋和选择性荧光机制有特殊的要求。在本文中，我们通过“三只鸟，一块石头”的策略开发了一种化学传感器，其中我们可以用一个配体检测三种金属离子。合成了一种高度敏感的基于偶氮官能化罗丹明的新型发光传感器，用于在红色发光区中的乙醇：H ₂ O介质中选择性荧光识别Al ³⁺，Cr ³⁺荧光和Cu ²⁺显色识别。在客体金属中，Cu ²⁺有效地抑制了发射，而Al ³⁺和Cr ³⁺通过螯合增强荧光（CHEF）和光诱导电子转移（PET）调控机制形成1：1络合物，诱导Al ^3+的发光增强4.76倍，Cr ³⁺增强2.47倍。通过复合物形成而受到限制的亚胺异构化会随着CHEF的瞬时发作而抑制正在进行的PET过程。该机理与NMR（¹ H和¹³ C），FT-IR，元素分析，DFT，TCSPC和pH依赖的研究相吻合。Cu 2 ⁺，Al ³⁺和Cr ^3+的微摩尔范围检测分别为1.1 µM，1.3 µM和1.5 µM ，易于渗透到HLC细胞中，更高的成像分辨率提高了其评估Al的潜力体外的³⁺和Cr ³⁺。此外，纸条的应用增加了其作为现场裸眼便携式固体探针的可行性。