Background

Spinal glioma is a nervous system tumor that tends to relapse and has no specific prognostic molecular biomarkers. Thus, a stable and reproduceable animal research model of spinal glioma is urgently needed.

New method

We established a new in situ tumor xenograft model of spinal glioma using nude mice. In this study, we implanted tumors into the cervical spinal cord of nude mice to mimic the pathological characteristics of the original tumors.

Results

Through anatomical experiments, we found that the cervical lamina of mice was thinner, the intervertebral space was much wider, and the adhesion muscles were more easily separated. According to the examination of spinal cord sections, the best puncture point we identified was located 0.9 mm lateral to the posterior median line at the level of the line between the midpoints of the scapulae and at a depth of 0.9 mm. In the nude mouse xenograft experiment, the implanted tumor tissue retained the pathological characteristics of the original tumor.

Comparison with existing method(s)

This model used the cervical spinal cord as the puncture site and patient-derived primary tumor cells, which has never been performed before. Tumor cells could be injected directly without damaging the lamina. Thus, we could reduce the risk of man-made spinal cord injury and infection and avoid destroying the stability and integrity of the spine.

Conclusions

This study established a stable and reliable animal model of spinal glioma for further molecular research and targeted therapy development.

中文翻译：

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脊柱神经胶质瘤的可再生原位异种移植模型。

背景

脊髓神经胶质瘤是一种神经系统肿瘤，容易复发，并且没有特定的预后分子生物标志物。因此，迫切需要一种稳定且可复制的脊髓神经胶质瘤动物研究模型。

新方法

我们使用裸鼠建立了新的脊柱神经胶质瘤原位肿瘤异种移植模型。在这项研究中，我们将肿瘤植入裸鼠的颈脊髓中，以模仿原始肿瘤的病理特征。

结果

通过解剖学实验，我们发现小鼠的颈椎更薄，椎间隙更宽，黏附肌肉更容易分离。根据对脊髓切片的检查，我们确定的最佳穿刺点位于后中线外侧0.9毫米，位于肩cap骨中点之间的线水平，深度为0.9毫米。在裸鼠异种移植实验中，植入的肿瘤组织保留了原始肿瘤的病理特征。

与现有方法的比较

该模型使用颈脊髓作为穿刺部位和患者来源的原发性肿瘤细胞，这是以前从未进行过的。肿瘤细胞可以直接注射而不会损害椎板。因此，我们可以减少人为脊髓损伤和感染的风险，并避免破坏脊柱的稳定性和完整性。

结论

这项研究建立了一个稳定可靠的脊柱神经胶质瘤动物模型，用于进一步的分子研究和靶向治疗的发展。