Nickel exposure causes hepato-renal toxicity via oxidative stress. Medicinal plants with antioxidants properties are being explored as treatment options. In this study, the effect of ethanol extract of Nigella sativa (ENS) on nickel chloride (NiCl2)-induced hepato-renal damage was evaluated by monitoring biochemical and oxidative stress markers. Additionally, the antioxidant capacity and phytochemical constituents of ENS were quantified using HPLC and GC-MS. NiCl2 significantly increased (p < 0.05) aspartate aminotransferase, creatinine, sodium ion, chloride ion and malondialdehyde levels, while antioxidant enzymes were decreased in the organs except for kidney glutathione-S-transferase when compared to the control. However, ENS exerted inhibitory effect against NiCl2 toxicity in both organs by reversing the biomarkers towards control levels. ENS has a high antioxidant capacity and is rich in antioxidants including gallic acid, quercetin, eucalyptol and levomenthol that may have accounted for the improvement of hepato-renal health in co-exposed rats. Our result suggests that amelioration of nickel chloride-induced hepato-renal pathology by ethanol extract of Nigella sativa was related to its antioxidant properties. Therefore, Nigella sativa could be valuable in the management of nickel-induced toxicity.

中文翻译：

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黑苜蓿的乙醇提取物具有抗氧化和改善氯化镍所致大鼠肝肾损伤的作用

镍暴露通过氧化应激引起肝肾毒性。具有抗氧化剂特性的药用植物正在被研究作为治疗选择。在这项研究中，通过监测生化和氧化应激标记物，评估了Nigella sativa（ENS）乙醇提取物对氯化镍（NiCl2）诱导的肝肾损伤的影响。另外，使用HPLC和GC-MS对ENS的抗氧化能力和植物化学成分进行了定量。与对照组相比，NiCl2显着增加（p <0.05）天冬氨酸转氨酶，肌酐，钠离子，氯离子和丙二醛水平，而除肾脏的谷胱甘肽S-转移酶外，其他器官的抗氧化酶均降低。但是，ENS通过将生物标记物逆转至对照水平，在两个器官中均发挥了对NiCl2毒性的抑制作用。ENS具有很高的抗氧化能力，并且富含抗氧化剂，其中包括没食子酸，槲皮素，桉树精和左薄荷脑，这可能有助于改善双暴露大鼠的肝肾健康。我们的研究结果表明，用Nigella sativa的乙醇提取物改善氯化镍诱导的肝肾病理与其抗氧化性能有关。因此，Nigella sativa在管理镍诱导的毒性方面可能是有价值的。