Phytohormones are the key factors regulating vascular development in plants, and they are also involved in tension wood (TW) formation. Although the theory of hormone distribution in TW formation is widely supported, the effects of endogenous hormones on TW formation have not yet been assessed. In this study, TW formation was induced in Catalpa bungei by artificial bending. The phytohormone content of TW, opposite wood (OW) and normal wood (NW) was determined using liquid chromatography-mass spectrometry (LC-MS), and transcriptome sequencing was performed. The hormone content and related gene expression data were comprehensively analyzed. The results of analyses of the plant hormone contents indicated significantly higher levels of cis-zeatin (cZ), indoleacetic acid (IAA) and abscisic acid (ABA) in TW than in OW. Genes involved in the IAA and ABA synthesis pathways, such as ALDH (evm.model.group5.1511) and UGT (evm.model.scaffold36.20), were significantly upregulated in TW. and the expression levels of ARF (evm.model.group5.1332), A-ARR (evm.model.group0.1600), and TCH4 (evm.model.group2.745), which participate in IAA, cZ and Brassinolide (BR) signal transduction, were significantly increased in TW. In particular, ARF expression may be regulated by long noncoding RNAs (lncRNAs) and the HD-ZIP transcription factor ATHB-15. We constructed a multiple hormone-mediated network of C. bungei TW formation based on hormone levels and transcriptional expression profiles were identified during TW formation.

中文翻译：

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全基因组分析Cat木中张力木材形成过程中lncRNA和mRNA表达以及内源激素调节。

植物激素是调节植物血管发育的关键因素，它们也参与张力木（TW）的形成。尽管激素在TW形成中的分布理论得到了广泛支持，但是尚未评估内源激素对TW形成的影响。在这项研究中，通过人工弯曲在Catalpa bungei中诱导了TW的形成。使用液相色谱-质谱法（LC-MS）测定TW，对立木材（OW）和普通木材（NW）的植物激素含量，并进行转录组测序。对激素含量及相关基因表达数据进行了综合分析。植物激素含量的分析结果表明，TW中的顺式玉米蛋白（cZ），吲哚乙酸（IAA）和脱落酸（ABA）显着高于OW。TW中，参与IAA和ABA合成途径的基因，如ALDH（evm.model.group5.1511）和UGT（evm.model.scaffold36.20），均显着上调。以及参与IAA，cZ和油菜素内酯的ARF（evm.model.group5.1332），A-ARR（evm.model.group0.1600）和TCH4（evm.model.group2.745）的表达水平（ BR）信号转导在TW中显着增加。特别是，ARF表达可能受长的非编码RNA（lncRNA）和HD-ZIP转录因子ATHB-15的调节。我们基于激素水平构建了多重激素介导的邦氏梭菌TW形成网络，并在TW形成过程中鉴定了转录表达谱。以及参与IAA，cZ和油菜素内酯的ARF（evm.model.group5.1332），A-ARR（evm.model.group0.1600）和TCH4（evm.model.group2.745）的表达水平（ BR）信号转导在TW中显着增加。特别是，ARF表达可能受长的非编码RNA（lncRNA）和HD-ZIP转录因子ATHB-15的调节。我们基于激素水平构建了多重激素介导的邦氏梭菌TW形成网络，并在TW形成过程中鉴定了转录表达谱。以及参与IAA，cZ和油菜素内酯的ARF（evm.model.group5.1332），A-ARR（evm.model.group0.1600）和TCH4（evm.model.group2.745）的表达水平（ BR）信号转导在TW中显着增加。特别是，ARF表达可能受长的非编码RNA（lncRNA）和HD-ZIP转录因子ATHB-15的调节。我们基于激素水平构建了多重激素介导的邦氏梭菌TW形成网络，并在TW形成过程中鉴定了转录表达谱。