Retinoblastoma is the commonest eye cancer occurring in the pediatric population. Circular RNAs (circRNAs) are essential regulators of tumorigenesis and development. The current experiment delves into the function and molecular basis of hsa_circ_0000034 in retinoblastoma progression. In the study, these series of experiments noted an upregulation of hsa_circ_0000034 in retinoblastoma cell lines and tissues. Retinoblastoma patients with raised hsa_circ_0000034 expressions were more likely to possess a more progressive International Integrated Reporting Council (IIRC) stage and optic nerve invasion. hsa_circ_0000034 knockdown caused a marked suppression in the proliferation and invasion of retinoblastoma cells in vitro. Mechanistically, hsa_circ_0000034 appeared to serve as a competitive endogenous RNA (ceRNA) in retinoblastoma through miR-361-3p sponging. In conclusion, our data proved that hsa_circ_0000034 promoted the oncogenicity of retinoblastoma via regulation of miR-361-3p expression, a finding that may contribute toward retinoblastoma therapeutics.

视网膜母细胞瘤是儿科人群中最常见的眼癌。环状RNA（circRNA）是肿瘤发生和发展的重要调节剂。当前的实验研究了hsa_circ_0000034在视网膜母细胞瘤进展中的功能和分子基础。在这项研究中，这些系列实验注意到视网膜母细胞瘤细胞系和组织中hsa_circ_0000034的上调。高表达hsa_circ_0000034的视网膜母细胞瘤患者更有可能具有更先进的国际综合报告委员会（IIRC）分期和视神经浸润。hsa_circ_0000034组合物在体外对视网膜母细胞瘤细胞的增殖和侵袭具有明显的抑制作用。机械上，hsa_circ_0000034似乎通过miR-361-3p海绵化在视网膜母细胞瘤中充当竞争性内源RNA（ceRNA）。总之，我们的数据证明hsa_circ_0000034通过调节miR-361-3p表达促进了成视网膜细胞瘤的致癌性，这一发现可能有助于成视网膜细胞瘤的治疗。