Salmonella enterica subsp. enterica serovar Abortusequi is a frequently reported pathogen causing abortion in mares. In this study, the preventive and therapeutic effects of phage P_IZ SAE-01E2 against S. Abortusequi in a mouse model of abortion were investigated. Phage P_IZ SAE-01E2 was stable at different temperatures (4 to 70°C) and pH values (pH 4 to 10) and could lyse the majority of the Salmonella serogroup O:4 and O:9 strains tested (25/28). There was no lysogeny-related, toxin, or antibiotic resistance-related gene in the genome of P_IZ SAE-01E2. All of these characteristics indicate that P_IZ SAE-01E2 has the potential for use in phage therapy. In in vivo experiments, 2 × 10³ CFU/mouse of S. Abortusequi ATCC 9842 was sufficient to lead to murine abortion (gestational day 14.5) within 48 h. A single intraperitoneal inoculation of P_IZ SAE-01E2 (10⁸ PFU/mouse, multiplicity of infection = 10⁵) 1 h before or after S. Abortusequi challenge provided effective protection to all pregnant mice (10/10). After 24 h of treatment with phage P_IZ SAE-01E2, the bacterial loads in both the placenta and the uterus of the infected mice were significantly decreased (<10² CFU/g) compared to those in the placenta and the uterus of the mice in the control group (>10⁶ CFU/g). In addition, the levels of inflammatory cytokines in the placenta and blood of the mice in the phage administration groups were significantly reduced (P < 0.05) compared to those in the placenta and blood of the mice in the control group. Altogether, these findings indicate that P_IZ SAE-01E2 shows the potential to block abortions induced by S. Abortusequi in vivo.

中文翻译：

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噬菌体PIZ SAE-01E2对马鼠流产沙门氏菌引起的流产的小鼠治疗作用。

肠沙门氏菌亚种。肠型血清型Abortusequi是引起母马流产的常见病原体。在这项研究中，噬菌体P _IZ SAE-01E2对S的预防和治疗作用。研究了流产小鼠模型中的Abortusequi。噬菌体P _IZ SAE-01E2在不同温度（4至70°C）和pH值（pH 4至10）下稳定，并可以裂解大多数沙门氏菌血清O：4和O：9菌株（25/28） 。P _IZ SAE-01E2的基因组中没有溶原相关，毒素或抗生素抗性相关的基因。所有这些特征表明，P _IZSAE-01E2具有用于噬菌体治疗的潜力。在体内实验中，2×10 ³的CFU /小鼠小号。Abortusequi ATCC 9842足以在48小时内导致小鼠流产（妊娠第14.5天）。在S之前或之后1小时一次腹膜内接种P _IZ SAE-01E2（10 ⁸ PFU /小鼠，感染复数= 10 ⁵）。Abortusequi攻击为所有妊娠小鼠提供了有效的保护（10/10）。用噬菌体P _IZ SAE-01E2处理24小时后，感染小鼠的胎盘和子宫中的细菌载量均显着降低（<10 ²CFU / g）与对照组小鼠的胎盘和子宫中的相比（> 10 ⁶ CFU / g）。另外，与对照组相比，噬菌体给药组小鼠的胎盘和血液中的炎性细胞因子水平显着降低（P < 0.05）。总而言之，这些发现表明P _IZ SAE-01E2显示了阻断由S诱导的流产的潜力。Abortusequi体内。