Adolescence is a period that exhibits both vulnerability and adaptation to environmental stimulus. This study explored the co‐existence effect of environmental enrichment (EE) and restraint stress (RS) on innate anxiety and depressive‐like behavior in adolescent mice. Male ICR mice were treated with daily EE and RS (4 h/d or 8 h/d) for 2 or 4 weeks from early adolescence (postnatal day 30) and emotional behaviors were evaluated 24 h after the end of treatment. 4 weeks of 8 h RS treatment decreased immobility time in forced swimming test, demonstrating an antidepressant‐like effect. For 2 weeks of treatment, 8 h RS significantly reduced the time spent in the lighted compartment of the light‐dark box, indicating an increased anxiety level. These results show that under the present experimental design, RS treatment with different duration could have different effect on mice emotion‐related behavior, but there was no interaction between EE and RS.

中文翻译：

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并发环境丰富和慢性束缚应激：对雄性青春期小鼠先天焦虑和抑郁样行为的影响

青春期是一个对环境刺激表现出脆弱性和适应能力的时期。本研究探讨了环境富集 (EE) 和约束应激 (RS) 对青春期小鼠先天焦虑和抑郁样行为的共存影响。从青春期早期（出生后第 30 天）开始，雄性 ICR 小鼠每天接受 EE 和 RS（4 小时/天或 8 小时/天）治疗 2 或 4 周，并在治疗结束后 24 小时评估情绪行为。4 周的 8 小时 RS 治疗减少了强迫游泳测试中的不动时间，证明了抗抑郁药的作用。在 2 周的治疗中，8 小时 RS 显着减少了在明暗盒的照明隔间中花费的时间，表明焦虑水平增加。这些结果表明，在目前的实验设计下，