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High-intensity ultrasound irradiation promotes the release of extracellular vesicles from C2C12 myotubes
Ultrasonics ( IF 4.2 ) Pub Date : 2021-02-01 , DOI: 10.1016/j.ultras.2020.106243
Noriaki Maeshige 1 , P Kent Langston 2 , Zhi-Min Yuan 3 , Hiroyo Kondo 4 , Hidemi Fujino 5
Affiliation  

Skeletal muscle is an important secretory organ in mammals, producing myriad chemical mediators ("myokines") with distinct biological action in different tissues, including anti-inflammatory activity. Extracellular vesicles (EVs) have recently been identified as a mode of myokine transport from muscle, facilitating such anti-inflammatory activity. In this report, we have demonstrated that high-intensity ultrasound (US) strongly induces EV secretion from cultured myotubes without a reduction in cell viability. High-intensity US of 3.0 W/cm2 with 20% duty cycle increased the number of EVs by 2-fold compared to control at 6 h. This effect was specific to EVs in the 100-150 nm size range. Thus, high-intensity US is a novel modality for inducing myocellular EV release and may hold therapeutic value.

中文翻译:

高强度超声照射促进 C2C12 肌管细胞外囊泡的释放

骨骼肌是哺乳动物的重要分泌器官,产生无数化学介质(“肌动蛋白”),在不同组织中具有不同的生物作用,包括抗炎活性。细胞外囊泡 (EVs) 最近被确定为肌肉肌因子转运的一种模式,促进了这种抗炎活性。在本报告中,我们已经证明高强度超声 (US) 强烈诱导培养的肌管分泌 EV,而不会降低细胞活力。与 6 小时的对照相比,具有 20% 占空比的 3.0 W/cm2 高强度 US 使 EV 数量增加了 2 倍。这种效应特定于 100-150 nm 尺寸范围内的 EV。因此,高强度超声是一种诱导肌细胞 EV 释放的新型方式,可能具有治疗价值。
更新日期:2021-02-01
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