In vitro studies have demonstrated that platelet lysate (PL) can serve as an alternative to platelet-rich plasma (PRP) to sustain chondrocyte proliferation and production of extracellular matrix components in chondrocytes. The present study aimed to evaluate the direct effects of PL on equine articular chondrocytes in vitro in order to provide a rationale for in vivo use of PL.

An in vitro cell proliferation and de-differentiation model was used: primary articular chondrocytes isolated from horse articular cartilage were cultured at low density under adherent conditions to promote cell proliferation. Chondrocytes were cultured in serum-free medium, 10% foetal bovine serum (FBS) supplemented medium, or in the presence of alginate beads containing 5%, 10% and 20% PL. Cell proliferation and gene expression of relevant chondrocyte differentiation markers were investigated. The proliferative capacity of cultured chondrocytes, was sustained more effectively at certain concentrations of PL as compared to that with FBS. In addition, as opposed to FBS, PL, particularly at percentages of 5% and 10%, could maintain the gene expression pattern of relevant chondrocyte differentiation markers. In particular, 5% PL supplementation showed the best compromise between chondrocyte proliferation capacity and maintenance of differentiation.

The results of the present study provide a rationale for using PL as an alternative to FBS for in vitro expansion of chondrocytes for matrix-assisted chondrocyte implantation, construction of 3D scaffolds for tissue engineering, and treatment of damaged articular cartilage.

体外研究表明，血小板溶解产物（PL）可以替代富含血小板的血浆（PRP），以维持软骨细胞增殖和软骨细胞中细胞外基质成分的产生。本研究旨在评估PL对体外马关节软骨细胞的直接作用，以便为PL在体内的使用提供依据。

使用体外细胞增殖和去分化模型：在贴壁条件下以低密度培养从马关节软骨分离的原发性软骨细胞，以促进细胞增殖。在无血清培养基，10％胎牛血清（FBS）补充培养基中或在含有5％，10％和20％PL的藻酸盐珠存在下培养软骨细胞。研究了相关软骨细胞分化标志物的细胞增殖和基因表达。与FBS相比，在一定的PL浓度下，培养的软骨细胞的增殖能力得到了更有效的维持。另外，与FBS相反，PL，特别是5％和10％的百分比，可以维持相关软骨细胞分化标记的基因表达模式。尤其是，

本研究的结果为使用PL替代FBS进行体外扩增软骨细胞进行基质辅助软骨细胞植入，组织工程的3D支架构建以及关节软骨受损的治疗提供了理论依据。