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Sulfakinin inhibits activity of digestive enzymes in the brown planthopper, Nilaparvata lugens
Journal of Asia-Pacific Entomology ( IF 1.5 ) Pub Date : 2020-09-06 , DOI: 10.1016/j.aspen.2020.09.004
Di Guo , Su Zhang , Yi-Jie Zhang , Jun-Yu Ma , Cong-Fen Gao , Shun-Fan Wu

In animals, feeding can regulate release of digestive enzymes. Digestive enzymes are produced and released in response to specific ratios of nutrients, so the quality and quantity of food ingested are important factors in the secretion and activity of digestive enzymes. In general, the enzyme activity and secretion in the fed insects are relatively higher than that in the unfed insects. Neuropeptides and peptide hormones are important regulators of enzyme activity. In several insects, the neuropeptide sulfakinin (SK) is known to be a regulator of feeding and digestion similar to cholecystokinin in mammals. However, the roles of diet and SK in regulation of activity of digestive enzymes in the important pest insect, the brown planthopper (Nilaparvata lugens), are unknown. In this study, we identified six genes encoding different digestive enzymes and cloned three of these. We found that enzymatic activity and transcriptional levels of digestive enzymatic activity genes were upregulated by refeeding animals for 5 h after 24 h starvation. Furthermore, injection of N. lugens SK reduces digestive enzyme activity and leads to a downregulation of digestive enzyme gene transcripts. This study provides new views into the action of diet and SK in regulation of digestive enzymes in (hemimetabolous) insects. Taken together with the roles of SK in inducing satiety, our data strongly suggest that SK signaling is important in regulation of food ingestion and processing.



中文翻译:

Sulfakinin抑制褐飞虱Nilaparvata lugens中消化酶的活性

在动物中,进食可以调节消化酶的释放。消化酶是根据营养素的特定比例产生和释放的,因此,所摄入食物的质量和数量是消化酶分泌和活性的重要因素。通常,被摄食昆虫中的酶活性和分泌相对高于未摄食昆虫中的酶活性和分泌。神经肽和肽激素是酶活性的重要调节剂。在几种昆虫中,与哺乳动物的胆囊收缩素相似,神经肽硫酸激酶(SK)已知是一种补给和消化的调节剂。然而,饮食和SK在重要害虫昆虫褐飞虱(Nilaparvata lugens)中调节消化酶活性的作用),未知。在这项研究中,我们鉴定了六个编码不同消化酶的基因,并克隆了其中的三个。我们发现饥饿24小时后再喂食动物5小时,酶活性和消化酶活性基因的转录水平被上调。此外,注射猪肺炎奈瑟氏球菌会降低消化酶的活性,并导致消化酶基因转录物的下调。这项研究提供了饮食和SK在(半代谢的)昆虫消化酶调控中的作用的新观点。结合SK在诱导饱腹感中的作用,我们的数据强烈表明SK信号在调节食物的摄取和加工中很重要。

更新日期:2020-09-06
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