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Effects of treadmill exercise on the regulation of tight junction proteins in aged mice.
Experimental Gerontology ( IF 3.9 ) Pub Date : 2020-09-06 , DOI: 10.1016/j.exger.2020.111077
Hyung Eun Shin 1 , Seong Eun Kwak 1 , Di Di Zhang 1 , Jihyun Lee 1 , Kyeong Jin Yoon 1 , Hae Sung Cho 1 , Hyo Youl Moon 2 , Wook Song 2
Affiliation  

Tight junction protein is representative regulator of gut permeability. Also, it has been noted for controlling inflammatory responses through tight junction. Therefore, in this study, we examined that whether tight junction protein is changed in aged mice, and to further, confirmed the effect of treadmill exercise on the tight junction protein. In in vitro study, doxorubicin that induces cell senescence was treated to Caco2 cells (colon cell) to mimic aging effect. After that, 5-aminoimidazole-4-carboxamide-1-β-D-ribofuranoside (AICAR), exercise mimic chemical that stimulates AMPK level, was also administered to Caco2 cells. In animal study, 2 months and 21 months C57BL/6 J mouse were treated with treadmill exercise for 4 weeks (YE = 5, OE = 5). Then, the tight junction protein expression level was examined by western blot. Also, serum lipopolysaccharide (LPS) and zonulin level were analyzed to identify gut permeability. In vitro studies showed that doxorubicin downregulates tight junction protein expression levels in Caco2 cell, and also AICAR treatment upregulates tight junction protein expression levels. In animal study, 4 weeks treadmill exercise upregulated claudin-1 (p < 0.05) and occludin (p < 0.01) protein expression level in 21 months old mice. Also, zonula occluden-1 (ZO-1) protein expression level was not significant difference among all mice group. In addition, old mice group had higher level of serum LPS compared to young mice group, but the level was downregulated in both 2 months and 21 months mice group after four weeks of treadmill exercise. Zonulin, which is known as degrading tight junction protein, is not significantly changed by both age and exercise. This study compared that tight junction protein expression level in old mice compared to its level in young mice, and also clarified that the effect of treadmill exercise on tight junction protein in both young and old mice.



中文翻译:

跑步机运动对衰老小鼠紧密连接蛋白的调节作用。

紧密连接蛋白是肠通透性的代表性调节剂。另外,已经注意到通过紧密连接来控制炎症反应。因此,在这项研究中,我们检查了老年小鼠中紧密连接蛋白是否发生改变,并且进一步证实了跑步机锻炼对紧密连接蛋白的影响。在体外研究中,将诱导细胞衰老的阿霉素处理至Caco2细胞(结肠细胞)以模拟衰老作用。此后,还将刺激AMPK水平的运动模拟化学物质5-氨基咪唑-4-羧酰胺-1-β-D-核呋喃糖苷(AICAR)应用于Caco2细胞。在动物研究中,分别在跑步机上锻炼2个月和21个月的C57BL / 6 J小鼠4周(YE = 5,OE = 5)。然后,通过蛋白质印迹检查紧密连接蛋白的表达水平。也,分析血清脂多糖(LPS)和zonulin水平以鉴定肠通透性。体外研究表明,阿霉素可下调Caco2细胞中紧密连接蛋白的表达水平,AICAR处理也可上调紧密连接蛋白的表达水平。在动物研究中,在跑步机上锻炼4周后,claudin-1(p  <0.05)和occludin(p  <0.01)蛋白在21个月大的小鼠中的表达水平。此外,在所有小鼠组中,小带闭合蛋白(ZO-1)的蛋白表达水平差异均无统计学意义。此外,老年小鼠组的血清LPS水平比年轻小鼠组高,但在跑步机运动四个星期后的2个月和21个月组中,血清LPS水平均被下调。Zonulin,被称为降解紧密连接蛋白,不会因年龄和运动而明显改变。这项研究将老年小鼠的紧密连接蛋白表达水平与年轻小鼠的紧密连接蛋白表达水平进行了比较,并且还阐明了跑步机运动对老年和老年小鼠的紧密连接蛋白的影响。

更新日期:2020-09-10
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