By comparing differentially abundant proteins and metabolites, the protein expression, metabolic changes and metabolic regulation mechanisms during the priming phase of liver regeneration (LR) were investigated. We combined proteomic analysis via isobaric tags for relative and absolute quantification (iTRAQ) with metabolomic analysis via nontargeted liquid chromatography-mass spectrometry (LC-MS). LC-MS was used to examine 29 energy metabolites expression alterations in targeted metabolomics. A total number of 441 differentially expressed proteins and 65 metabolites were identified. PSMB10, PSMB5, RCG_63409, PSME4 and PSMB7 were key node proteins, these proteins are involved in the proteasome pathway. The most strongly enriched transcription factor motif was TP63. These results point out a critical role of the proteasome pathway (defense mechanisms) and of TP63 (metabolic regulator) as the key transcription factor during the priming phase of LR. Metabolomic and metabolite analysis showed that profiling indicates upregulation of arginine biosynthesis and glycolysis as the main ATP-delivering pathway. Integrative proteomic and metabolomic analysis showed that biomolecular changes were primarily related to the neurological disease, cell death and survival and cell morphology. What's more, neurotransmitters may play an important role in the regulation of LR.

通过比较差异丰富的蛋白质和代谢产物，研究了肝脏再生（LR）启动阶段的蛋白质表达，代谢变化和代谢调控机制。我们将通过等压变量标签进行的蛋白质组学分析相对和绝对定量（iTRAQ）与通过无目标液相色谱-质谱（LC-MS）进行的代谢组学分析相结合。LC-MS用于检查靶向代谢组学中29种能量代谢物的表达变化。总共鉴定出441种差异表达的蛋白质和65种代谢产物。PSMB10，PSMB5，RCG_63409，PSME4和PSMB7是关键节点蛋白，这些蛋白都参与了蛋白酶体途径。浓缩程度最高的转录因子基序是TP63。这些结果指出了蛋白酶体途径（防御机制）和TP63（代谢调节剂）在LR启动阶段的关键转录因子的关键作用。代谢组学和代谢物分析表明，分析表明精氨酸的生物合成和糖酵解是上调ATP的主要途径。综合蛋白质组学和代谢组学分析表明，生物分子的变化主要与神经系统疾病，细胞死亡和存活以及细胞形态有关。而且，神经递质可能在LR的调节中起重要作用。代谢组学和代谢产物分析表明，分析表明精氨酸的生物合成和糖酵解是上调ATP的主要途径。综合蛋白质组学和代谢组学分析表明，生物分子的变化主要与神经系统疾病，细胞死亡和存活以及细胞形态有关。此外，神经递质可能在LR的调节中起重要作用。代谢组学和代谢产物分析表明，分析表明精氨酸的生物合成和糖酵解是上调ATP的主要途径。综合蛋白质组学和代谢组学分析表明，生物分子的变化主要与神经系统疾病，细胞死亡和存活以及细胞形态有关。而且，神经递质可能在LR的调节中起重要作用。