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In Vitro Biological Characterization of Natural Hydroxyapatite/Single-Walled Carbon Nanotube Composite Coatings Synthesized by Electrophoretic Deposition on NiTi Shape Memory Alloy
Journal of Materials Engineering and Performance ( IF 2.3 ) Pub Date : 2020-09-05 , DOI: 10.1007/s11665-020-05094-0
Leila Nematzadeh , Nazila Horandghadim , Vida Khalili , Jafar Khalil-Allafi

In the current work, the composite coating of natural hydroxyapatite (nHA)/single-walled carbon nanotubes (SWCNTs) with various contents of SWCNTs (0, 0.5, 1, and 2 wt.%) was applied on NiTi using electrophoretic deposition (EPD). Before the deposition process, the nanotubes were functionalized following the chemical oxidation method and characterized by FTIR. The sintering of samples was conducted at 800 °C for 3 h in a tube furnace under an argon atmosphere. The surface and cross-sectional microstructure of coatings was studied using a scanning electron microscope. The x-ray diffraction was utilized to investigate the effect of the SWCNTs secondary phase on the phase composition of the nHA layer after sintering. The incorporation of SWCNTs into the nHA layer resulted in the decomposition of some HA into the β-tricalcium phosphate phase. The tensile test was applied and displayed the enhancement in adhesion strength of nHA coating from 17.2 to 25 MPa after composing with 2 wt.% SWCNTs. The wettability of surfaces was assessed by measuring DMEM cell culture contact angles. The appraisement of the Ni ions release from the substrate demonstrated that the lowest release of Ni ions into DMEM cell culture after 7 days of incubation is achieved from NiTi coated with nHA-1 wt.% SWCNTs. The in vitro biocompatibility of the samples was pursued by MG63 osteoblast cell culturing and MTT assay. The highest cell attachment and proliferation on nHA-1 wt.% SWCNTs coating were attributed to the least toxic Ni ions release from the substrate of that.



中文翻译:

在NiTi形状记忆合金上电泳沉积合成天然羟基磷灰石/单壁碳纳米管复合涂层的体外生物学特性

在当前工作中,使用电泳沉积(EPD)在NiTi上涂覆了天然羟基磷灰石(nHA)/单壁碳纳米管(SWCNT)和不同含量的SWCNT(0、0.5、1和2 wt。%)的复合涂层。 )。在沉积过程之前,按照化学氧化方法对纳米管进行功能化,并通过FTIR对其进行表征。样品的烧结在氩气气氛下在管式炉中于800°C进行3 h。使用扫描电子显微镜研究了涂层的表面和截面微观结构。利用X射线衍射研究了烧结后SWCNTs第二相对nHA层相组成的影响。将SWCNT掺入nHA层中导致一些HA分解成β-磷酸三钙相。施加拉伸试验并显示出在用2重量%的SWCNT组成后,nHA涂层的粘合强度从17.2提高到25MPa。通过测量DMEM细胞培养物的接触角来评估表面的润湿性。从底物释放的镍离子的评估表明,孵育7天后,从涂覆有nHA-1 wt。%SWCNT的NiTi可以实现最低的Ni离子向DMEM细胞培养的释放。样品的体外生物相容性通过MG63成骨细胞培养和MTT测定法进行。nHA-1 wt。%SWCNTs涂层上最高的细胞附着和增殖归因于从其底物释放的毒性最低的Ni离子。通过测量DMEM细胞培养物的接触角来评估表面的润湿性。从底物释放的镍离子的评估表明,孵育7天后,从涂覆有nHA-1 wt。%SWCNT的NiTi可以实现最低的Ni离子向DMEM细胞培养的释放。样品的体外生物相容性通过MG63成骨细胞培养和MTT测定法进行。nHA-1 wt。%SWCNTs涂层上最高的细胞附着和增殖归因于从其底物释放的毒性最低的Ni离子。通过测量DMEM细胞培养物的接触角来评估表面的润湿性。从底物释放的镍离子的评估表明,孵育7天后,从涂覆有nHA-1 wt。%SWCNT的NiTi可以实现最低的Ni离子向DMEM细胞培养的释放。样品的体外生物相容性通过MG63成骨细胞培养和MTT测定法进行。nHA-1 wt。%SWCNTs涂层上最高的细胞附着和增殖归因于从其底物释放的毒性最低的Ni离子。样品的体外生物相容性通过MG63成骨细胞培养和MTT测定法进行。nHA-1 wt。%SWCNTs涂层上最高的细胞附着和增殖归因于从其底物释放的毒性最低的Ni离子。样品的体外生物相容性通过MG63成骨细胞培养和MTT测定法进行。nHA-1 wt。%SWCNTs涂层上最高的细胞附着和增殖归因于从其底物释放的毒性最低的Ni离子。

更新日期:2020-09-07
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