当前位置: X-MOL 学术J. Alzheimer’s Dis. › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Microglia Demonstrate Local Mixed Inflammation and a Defined Morphological Shift in an APP/PS1 Mouse Model.
Journal of Alzheimer’s Disease ( IF 4 ) Pub Date : 2020-09-04 , DOI: 10.3233/jad-200098
Olivia G Holloway 1 , Anna E King 1 , Jenna M Ziebell 1
Affiliation  

Background:Microglia are traditionally described as the immune cells of the brain and have an inflammatory role in Alzheimer’s disease (AD). Microglial morphological and phenotypic shifts in AD have not been fully characterized; however, microglia are often described as either pro- or anti-inflammatory. Objective:To determine microglial if microglial morphology and phenotype changes with disease status. Methods:This study observed morphology through Iba1 immunohistochemistry on tissue sections encompassing the primary motor cortex and somatosensory barrel fields. Immunohistochemistry for pro-inflammatory markers: CD14 and CD40; and anti-inflammatory markers: CD16 and TREM2, was performed at 3, 6, and 12 months of age which correlated with pre-plaque, onset, and significant plaque load in APP/PS1 brains (n = 6) and compared to age-matched littermate controls (n = 6). Results:Microglia demonstrated a defined morphological shift with time. Deramified morphologies increased in the APP/PS1, at both 6 months (p < 0.0001) and 12 months (p < 0.0001). At 12 months, there were significantly lower numbers of ramified microglia (p < 0.001). Results indicated that microglia have a heterogenic marker immunoreactivity as CD16, TREM2, and CD40 were associated with an activated morphology at the same time points. All inflammatory markers were significantly upregulated at 12 months in the APP/PS1 mice (TREM2 (F (2,30) = 10.75, p = 0.0003), CD40 (F (2,30) = 15.86, p < 0.0001), CD14 (F (2,30) = 6.84, p = 0.0036), and CD16 (F (2,30) = 3.026, p = 0.0635)). Conclusion:Our data indicate that pro- and anti-inflammatory factors of microglia occur in APP/PS1 mice.

中文翻译:

小胶质细胞在 APP/PS1 小鼠模型中表现出局部混合炎症和明确的形态学转变。

背景:小胶质细胞传统上被描述为大脑的免疫细胞,在阿尔茨海默病 (AD) 中具有炎症作用。AD 中的小胶质细胞形态和表型变化尚未完全表征;然而,小胶质细胞通常被描述为促炎或抗炎。目的:确定小胶质细胞的形态和表型是否随疾病状态发生变化。方法:本研究通过 Iba1 免疫组化对包括初级运动皮层和躯体感觉桶区域的组织切片进行形态学观察。促炎标志物的免疫组织化学:CD14 和 CD40;和抗炎标记物:CD16 和 TREM2,在 3、6 和 12 个月大时进行,与斑块前、发病、APP/PS1 大脑中的显着斑块负荷(n = 6)并与年龄匹配的同窝对照(n = 6)相比。结果:小胶质细胞随着时间表现出明确的形态变化。在 6 个月 (p < 0.0001) 和 12 个月 (p < 0.0001) 时,APP/PS1 中的脱链形态增加。在 12 个月时,分枝小胶质细胞的数量显着减少(p < 0.001)。结果表明小胶质细胞具有异源标记免疫反应性,因为 CD16、TREM2 和 CD40 在同一时间点与激活的形态相关。所有炎症标志物在 12 个月时在 APP/PS1 小鼠中显着上调(TREM2 (F (2,30) = 10.75, p = 0.0003)、CD40 (F (2,30) = 15.86, p < 0.0001)、CD14 ( F (2,30) = 6.84, p = 0.0036) 和 CD16 (F (2,30) = 3.026, p = 0.0635))。结论:
更新日期:2020-09-05
down
wechat
bug