Purpose: The management of pain and inflammation with non-steroidal anti-inflammatory drugs and opioid analgesics are currently encountering severe adverse reactions. To overcome these problems, herbal remedies may offer new alternative medicines. Hygrophila schulli is a medicinal plant traditionally used for the treatment of pain and inflammation-related disorders; yet, these claims are not scientifically validated. Hence, this study was aimed to validate the traditional use of Hygrophila schulli leaves as anti-inflammatory and analgesic remedy.
Methods: In vitro anti-hyaluronidase assay and in vivo carrageenan-induced hind paw oedema model were used to evaluate the anti-inflammatory property of ethanolic leaf extract of Hygrophila schulli. Tail immersion and acetic acid-induced writhing tests were performed to determine the central and peripheral analgesic activity of the leaf extract, respectively.
Results: The ethanolic leaf extract exhibited significant anti-hyaluronidase activity (P< 0.001) and significant inhibition of carrageenan-induced paw oedema (P< 0.05) compared to untreated controls. Similarly, the extract significantly prolonged the reaction time of mice (P< 0.05) for the hot-water stimuli. Furthermore, an oral dose of the extract showed significant inhibition (P< 0.01) of acetic acid-induced abdominal contractions of mice. Besides, the ethanolic leaf extract did not cause any obvious sign of acute toxicity at a single oral dose of 2 g/kg.
Conclusion: The findings of this study may partially support the acclaimed traditional use of Hygrophila schulli leaves for the treatment of pain and inflammatory conditions.

Keywords: pain, inflammation, carrageenan, hyaluronidase, Hygrophila schulli


中文翻译：

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Hygrophila schulli 叶子的抗伤害和抗炎活性。

目的：使用非甾体抗炎药和阿片类镇痛药治疗疼痛和炎症目前正遇到严重的不良反应。为了克服这些问题，草药可能会提供新的替代药物。Hygrophila schulli是一种传统上用于治疗疼痛和炎症相关疾病的药用植物；然而，这些说法未经科学验证。因此，本研究旨在验证Hygrophila schulli叶子作为抗炎和镇痛药物的传统用途。
方法：采用体外抗透明质酸酶试验和体内角叉菜胶诱导的后爪水肿模型，评价叶乙醇提取物的抗炎作用。嗜水菌。进行尾部浸泡和乙酸诱导的扭体试验以确定叶提取物的中枢和外周镇痛活性，分别。
结果：与未处理的对照组相比，乙醇叶提取物表现出显着的抗透明质酸酶活性（P < 0.001）和对角叉菜胶引起的爪水肿的显着抑制（P < 0.05）。同样，提取物显着延长了小鼠对热水刺激的反应时间（ P < 0.05）。此外，口服剂量的提取物显示出显着的抑制作用（P< 0.01) 乙酸诱导的小鼠腹部收缩。此外，乙醇叶提取物在单次口服剂量为 2 g/kg 时没有引起任何明显的急性毒性迹象。
结论：这项研究的结果可能部分支持广受赞誉的传统使用Hygrophila schulli叶子治疗疼痛和炎症的做法。

关键词：疼痛，炎症，角叉菜胶，透明质酸酶，Hygrophila schulli