Life-threatening bacterial infections are a major concern in health care services worldwide. This retrospective study aimed to demonstrate genetic and biochemical diversity in isolates of Acinetobacter baumannii and Pseudomonas aeruginosa from a public hospital in Brazil. A total of 63 isolates collected from different sites of infection and hospital sectors were characterized, and their susceptibility profile to antibiotics was assessed for 18 drugs belonging to 8 antimicrobial categories using the automated BACTEC system. Genetic diversity was assessed using the multiple locus variable number tandem repeat analysis. Among the isolates of A. baumannii, 83% were classified as extensively drug resistant (XDR), and 17 genotypic profiles were identified. About 67% of P. aeruginosa isolates were susceptible to antimicrobials and were distributed into 37 genotypic profiles, revealing genetic heterogeneity. This study has demonstrated the multicolonization of investigated pathogens and the high frequency (95.8%) of multidrug-resistant and XDR, as well as high genetic diversity, among the isolates supporting the continuous need to monitor these species in the hospital environment.

中文翻译：

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巴西某公立医院临床鲍曼不动杆菌和铜绿假单胞菌的遗传和生化多样性

危及生命的细菌感染是全球卫生保健服务中的一个主要问题。这项回顾性研究旨在证明来自巴西一家公立医院的鲍曼不动杆菌和铜绿假单胞菌分离株的遗传和生化多样性。对从不同感染地点和医院部门收集的总共 63 株分离株进行了表征，并使用自动化 BACTEC 系统评估了属于 8 个抗菌类别的 18 种药物的抗生素敏感性。使用多基因座可变数目串联重复分析评估遗传多样性。在A. baumannii的分离株中, 83% 被归类为广泛耐药 (XDR)，并确定了 17 种基因型谱。大约 67% 的铜绿假单胞菌分离株对抗生素敏感，并分布在 37 个基因型谱中，揭示了遗传异质性。该研究表明，在支持在医院环境中持续监测这些物种的分离株中，所研究病原体的多重定殖、多重耐药性和 XDR 的高频率 (95.8%) 以及高遗传多样性。