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A Terpyridine Zinc Complex for Selective Detection of Lipid Pyrophosphates: A Model System for Monitoring Bacterial O- and N-Transglycosylations.
The Journal of Organic Chemistry ( IF 3.6 ) Pub Date : 2020-09-04 , DOI: 10.1021/acs.joc.0c01252
Tse-Wei Hsu,Hsin-Chuan Hsu,Hsin-Yu Chan,Jim-Min Fang

To develop an effective method for probing O- and N-glycosyltransfer reactions that are accompanied by the release of undecaprenyl pyrophosphate, solanesyl pyrophosphate (SPP) is used as a surrogate to bind a terpyridine zinc complex (Tpy-Zn), forming a fluorescent [Tpy-Zn]-SPP complex (Kass 106,000 M–1 in EtOH-CHCl3) with 5.8 μM LOD in HEPES buffer (10 mM, pH 7.4) containing 10 mM CaCl2 and 0.08% decyl PEG, which is similar to the bioassay conditions for lipid II polymerization.

中文翻译:

选择性吡啶脂质焦磷酸的特吡啶吡啶锌配合物:监测细菌O-和N-转糖基化的模型系统。

为了开发一种有效的方法来探测伴有十一碳烯基焦磷酸盐释放的O-和N-糖基转移反应,使用茄基焦磷酸盐(SPP)作为替代物来结合一个联吡啶锌配合物(Tpy-Zn),形成一种荧光[ Tpy-Zn] -SPP络合物(EtOH-CHCl 3中的K106,000 M –1),在含有10 mM CaCl 2和0.08%癸基PEG的HEPES缓冲液(10 mM,pH 7.4)中具有5.8μMLOD。脂质II聚合的生物测定条件。
更新日期:2020-10-02
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