Zinc is an essential trace element for animals in several biological processes, particularly in energy production, and it is acquired from food ingestion. In this context, a microplate-based fluorimetric assay was developed for simple, fast, and low-cost determination of zinc in pet food using 2,2′-((4-(2,7-difluoro-3,6-dihydroxy-4aH-xanthen-9-yl)-3-methoxyphenyl)azanediyl)diacetic acid (FluoZin-1) as fluorescent probe. Several aspects were studied, namely, the stability of the fluorescent product over time, the FluoZin-1 concentration, and the pH of reaction media. The developed methodology provided a limit of detection of 1 μg L⁻¹ in sample acid digests, with a working range of 10 to 200 μg L⁻¹, corresponding to 100–2000 mg of Zn per kg of dry dog food samples. Intraday repeatability and interday repeatability were assessed, with relative standard deviation values < 3.4% (100 μg L⁻¹) and <11.7% (10 μg L⁻¹). Sample analysis indicated that the proposed fluorimetric assay provided results consistent with ICP-MS analysis. These results demonstrated that the developed assay can be used for rapid determination of zinc in dry dog food.

中文翻译：

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微型荧光法定量干狗粮中的锌。

锌是几种生物过程中动物必不可少的微量元素，尤其是在能源生产中，它是从食物中摄取的。在这种情况下，开发了一种基于微孔板的荧光测定法，可使用2,2'-（（（4-（2,7-difluoro-3,6-dihydroxy- 4a H-黄嘌呤-9-基）-3-甲氧基苯基）氮杂二基）二乙酸（FluoZin-1）作为荧光探针。研究了几个方面，即荧光产物随时间的稳定性，FluoZin-1浓度和反应介质的pH。该改进的方法提供的检测1个的极限 μ克L- ^-1在样品酸消化，用10至200的工作范围 μ克L- ^-1，相当于每千克干狗粮样品中100-2000毫克的锌。盘中的可重复性和可重复性日间进行了评估，相对标准偏差值<3.4％（100  μ克L- ^-1）和<11.7％（10  μ克L- ^-1）。样品分析表明，所提出的荧光测定法提供的结果与ICP-MS分析相符。这些结果表明，开发的测定方法可用于快速测定狗干粮中的锌。