Northern yellow bats (Dasypterus intermedius) are tree-roosting bats in the family Vespertilionidae comprised of two subspecies: D. intermedius intermedius and D. intermedius floridanus. The two subspecies are thought to be geographically separated. Due to their cryptic morphology, this hypothesis has never been tested and can benefit from being examined within a molecular framework. In this study, mitochondrial sequence data from 38 D. intermedius and nuclear sequence data from 14 D. intermedius from across their range were used to test the hypothesis that genetically defined groups correspond geographically with the two morphologically defined subspecies. Although high levels of divergence of the mitochondrial sequence (11.6%) suggest genetically distinct clusters sympatric in southern Texas, no genetic structure was recovered with the nuclear marker. Moreover, the mitochondrial sequence data recovered a paraphyletic relationship between the two subspecies of D. intermedius with the Cuban yellow bat (D. insularis), whereas no such paraphyly was recovered from analysis of the nuclear marker. Divergence time based on analyses of mitochondrial sequence for the two subspecies was approximately 5.5 Ma. The patterns observed are hypothesized to be the result of past isolation of lineages and secondary contact that is currently contributing to gene flow.

中文翻译：

---

系统地理学分析揭示了 Dasypterus intermedius 的线粒体核不一致性

北方黄蝙蝠 (Dasypterus intermedius) 是 Vespertilionidae 科的树栖蝙蝠，由两个亚种组成：D. intermedius intermedius 和 D. intermedius floridanus。这两个亚种被认为在地理上是分开的。由于其神秘的形态，这个假设从未被测试过，可以从分子框架内的检查中受益。在这项研究中，来自 38 D. intermedius 的线粒体序列数据和来自其范围内的 14 D. intermedius 的核序列数据用于测试遗传定义的群体在地理上与两个形态学定义的亚种相对应的假设。尽管线粒体序列的高度差异 (11.6%) 表明在德克萨斯州南部存在遗传上不同的同域簇，用核标记没有恢复到遗传结构。此外，线粒体序列数据恢复了中间 D. intermedius 的两个亚种与古巴黄蝙蝠 (D. insularis) 之间的并系关系，而从核标记分析中没有发现这种并系。基于对两个亚种线粒体序列分析的发散时间约为 5.5 Ma。观察到的模式被假设是过去谱系隔离和目前促进基因流动的二次接触的结果。基于对两个亚种线粒体序列分析的发散时间约为 5.5 Ma。观察到的模式被假设是过去谱系隔离和目前促进基因流动的二次接触的结果。基于对两个亚种线粒体序列分析的发散时间约为 5.5 Ma。观察到的模式被假设是过去谱系隔离和目前促进基因流动的二次接触的结果。