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Mucin histochemistry as a tool to assess rostral digestive tract health in a teleost model (Danio rerio).
Journal of Fish Diseases ( IF 2.5 ) Pub Date : 2020-09-03 , DOI: 10.1111/jfd.13252
Sanet H Kotzé 1 , Ann Huysseune 2
Affiliation  

The zebrafish (Danio rerio), a small agastric teleost fish, has become a popular species to model diseases of the gastrointestinal tract. Remarkably, its rostral digestive tract has been largely understudied. To allow for accurate reporting and to improve the translational potential of this research model, the present study first proposes terminology for the different regions of the rostral digestive tract in zebrafish. Based on markers (in particular mucin histochemistry) which are easy to apply in routine histopathology laboratories, five regions are identified, in line with descriptions for carp, a close relative of zebrafish. The mucin histochemical staining is specific and distinct in each region identified, and can be used as a tool to assess health of the rostral digestive tract. In addition, we identify a transitional zone between the oesophagus and intestinal bulb which may represent a remnant of a rudimentary stomach.

中文翻译:

Mucin组织化学作为评估硬骨动物模型(Danio rerio)眼部消化道健康的工具。

斑马鱼(Danio rerio)是一种小型硬骨鱼类,已经成为模拟胃肠道疾病的流行物种。值得注意的是,它的延髓消化道已被大大研究。为使报告准确并提高该研究模型的翻译潜力,本研究首先提出了斑马鱼表皮消化道不同区域的术语。基于易于在常规组织病理学实验室中应用的标记物(特别是粘蛋白组织化学),根据鲤鱼(斑马鱼的近亲)的描述,确定了五个区域。粘蛋白组织化学染色在鉴定的每个区域都是特异性的和不同的,并且可以用作评估眼部消化道健康的工具。此外,
更新日期:2020-11-06
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