A group of pathogenic nucleocytoplasmic large DNA viruses (NCLDVs) related to the Mimiviridae family infect farmed sturgeons across Europe, causing mild‐to‐severe losses. One of these viruses, Acipenser iridovirus‐European (AcIV‐E), was identified in six sturgeon species. During the 2018–2019 period, nine sick Siberian (A. baerii) and Russian (A. gueldenstaedtii) sturgeons were sampled in Ukrainian farms and tested for the presence of AcIV‐E using real‐time PCR. The presence of AcIV‐E was confirmed in some samples. High‐resolution melting (HRM) assay and Sanger sequencing demonstrated the presence in three farms of two alleles of the major capsid protein (MCP) gene, called var1 and var2. Five samples carried both var1 and var2 at varying ratios, and the sixth sample was infected with only var1. These results constitute the first detection of AcIV‐E in Ukraine and the first detection of a sample carrying only var1. The full‐length sequences of the MCP genes confirmed the existence of two genetic lineages of AcIV‐E, tentatively named V1 and V2, each displaying multiple substitutions in the MCP gene. Some of the MCP sequences showed a genetic relationship to both V1 and V2 lineages, depending on the fragment examined. Most likely, these sequences resulted from recombination events.

中文翻译：

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Ukraine鱼mimiviruses在乌克兰的第一个遗传特征。

与Mimiviridae家族有关的一组致病性核质大DNA病毒（NCLDV）感染了欧洲各地的养殖st鱼，造成了轻度至重度损失。在六个six鱼物种中鉴定出其中一种病毒，即Acipenser iridovirus -European（AcIV-E）。在2018-2019年期间，在乌克兰农场采样了九只患病的西伯利亚（（A. baerii）和俄罗斯（（A. gueldenstaedtii）ons鱼，并使用实时PCR检测了AcIV-E的存在。在某些样品中证实了AcIV-E的存在。高分辨率熔解（HRM）分析和Sanger测序证明在三个场中存在两个主要等衣壳蛋白（MCP）基因的等位基因，分别为var1和var2。五个样本都以不同的比率携带var1和var2，而第六个样本仅感染var1。这些结果构成了乌克兰首次检测到AcIV-E，并且首次检测到仅携带var1的样品。MCP基因的全长序列证实了AcIV-E的两个遗传谱系的存在，暂定名为V1和V2，每个谱系在MCP基因中显示出多个取代。根据所检查的片段，某些MCP序列与V1和V2谱系均显示出遗传关系。这些序列很可能是重组事件产生的。