This study reports the green synthesis and urease inhibitory activities of Ag and Au nanoparticles (NPs) using Crataegus oxyacantha extract. The synthesized NPs were characterized by UV–visible, FT-IR spectroscopy, atomic force microscopy, and scanning electron microscopy. The obtained NPs were spherical in shape, and their size was around 85 nm. A strong correlation between the phytochemicals present in the extract and their capability for the synthesis of NPs was observed. Furthermore, the shape, size, stability, and bioactivity of the NPs were strongly influenced by the stabilizing phytochemicals. The experimental analysis suggested that these NPs have substantial stability in a diverse range of physiological conditions such as pH, salinity, and temperature. The NPs exhibited potent urease enzyme inhibitory activities with percent inhibition of 99.25 and IC₅₀ value of 1.38 ± 0.3, comparable to the standard (thiourea percent inhibition, that is, 98.2% and IC₅₀ value 5.3 ± 0.04). These results suggested that the proposed NPs could be used in the homeopathic and pharmaceutical industries for biomedical applications.

中文翻译：

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山楂提取物绿色合成银和金纳米粒子及其脲酶抑制活性

本研究报告了使用山楂的 Ag 和 Au 纳米粒子 (NPs) 的绿色合成和脲酶抑制活性提炼。合成的纳米颗粒通过紫外-可见光、傅里叶变换红外光谱、原子力显微镜和扫描电子显微镜进行表征。获得的纳米颗粒呈球形，尺寸约为 85 nm。观察到提取物中存在的植物化学物质与其合成 NP 的能力之间存在很强的相关性。此外，NPs 的形状、大小、稳定性和生物活性受到稳定植物化学物质的强烈影响。实验分析表明，这些纳米颗粒在各种生理条件（如 pH 值、盐度和温度）中都具有相当大的稳定性。NPs 表现出强大的脲酶抑制活性，抑制率为 99.25 和 IC ₅₀值 1.38 ± 0.3，与标准相当（硫脲抑制百分比，即 98.2% 和 IC ₅₀值 5.3 ± 0.04）。这些结果表明，所提出的 NPs 可用于顺势疗法和制药行业的生物医学应用。