Sustainable sources of energy are sought because of the finite nature of nonrenewable resources. Renewable energy from the lignocellulosic biomass of the forest ecosystem reduces dependency on nonrenewable resources to overcome the global energy crisis. In this study, we evaluated the physicochemical parameters of, and enzymatic activity in, 25 forest soil samples collected from Thandikudi (Western Ghats) and the Sirumalai Hills (Eastern Ghats) in Tamil Nadu, India. Soil‐sample analysis revealed that two samples obtained from the Western Ghats exhibited low and high lignocellulolytic enzyme activity, respectively, and they were considered for further study. Sample A showed low lignocellulolytic enzyme activity, whereas sample B showed high lignocellulolytic enzyme activity. The variation in the soil enzymes led us to determine the microbial communities in these two soil samples using nanopore sequencing, which targeted the V1–V9 regions of 16S rDNA. The results highlighted the positive correlation between physicochemical properties and soil enzymes. Principal component analysis revealed that, among the soil enzymes, saccharase and β‐glucosidase predominantly influenced lignocellulosic biomass degradation. The nanopore sequencing results showed that, among the microbes present, the percentage of Firmicutes was higher in soil sample B (23.03%) compared with soil sample A (14.90%). Among the bacterial genera, Bacillus was abundant in these two soil samples followed by Pseudomonas, Escherichia, and Klebsiella. The variation in the occurrence of enzymes and the availability of nutrients in the two soil samples had a positive effect on the abundance of these microbial genera. Moreover, the plentiful presence of Bacillus in soil sample B indicates the richness of lignocellulose. © 2020 Society of Chemical Industry and John Wiley & Sons, Ltd

中文翻译：

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阐明了印度泰米尔纳德邦东西高止山脉森林土壤中降解木质纤维素生物质的微生物多样性和木质纤维素分解酶

由于不可再生资源的有限性，寻求可持续的能源。来自森林生态系统木质纤维素生物质的可再生能源减少了对不可再生资源的依赖，从而克服了全球能源危机。在这项研究中，我们评估了从印度泰米尔纳德邦的Thandikudi（西高止山脉）和Sirumalai Hills（东高止山脉）收集的25种森林土壤样品的理化参数和酶活性。土壤样品分析表明，从西高止山脉获得的两个样品分别表现出低和高的木质纤维素分解酶活性，并被考虑作进一步研究。样品A显示低的木质纤维素分解酶活性，而样品B显示高的木质纤维素分解酶活性。土壤酶的变化使我们能够使用纳米孔测序法来确定这两个土壤样品中的微生物群落，其靶向16S rDNA的V1-V9区。结果强调了理化性质与土壤酶之间的正相关性。主成分分析表明，在土壤酶中，蔗糖酶和β-葡萄糖苷酶主要影响木质纤维素生物质的降解。纳米孔测序结果表明，在存在的微生物中，土壤样品B中的Firmicutes百分比（23.03％）高于土壤样品A（14.90％）。在细菌属中，这两个土壤样品中的芽孢杆菌含量很高，其次是假单胞菌，大肠埃希氏菌和克雷伯菌。两个土壤样品中酶的发生和养分有效性的变化对这些微生物属的丰度具有积极影响。而且，芽孢杆菌的存在土壤样品B中的B表示木质纤维素的丰富度。©2020年化学工业协会和John Wiley＆Sons，Ltd