Serological diagnosis of Bartonella henselae infection mainly rely on microscopic immunofluorescence assays (IFA), which are however time-consuming and poorly standardized. The aim of the study was to assess the use of the new fully automated VirClia® chemiluminescent immunoassays for the detection of IgG and IgM anti-B. henselae antibodies. 81 patients with a well-defined B. henselae infection as well as 80 patients with an alternative disease were included. The VirClia® IgG antibody assay showed a sensitivity of 79.0% and a specificity of 93.8% for the diagnosis of B. henselae infection. For the VirClia® IgM assay, results were more conflicting with a sensitivity of 42.0% and a specificity of 98.2% to predict IFA IgM results. In 11 additional patients with uninterpretable IFA due to autoimmune antibodies, VirClia® assays were able to deliver valuable quantitave results. The VirClia® IgG assay shows good analytical and clinical performances and could be easily integrated in the diagnostic workflow of B. henselae infection.

汉赛巴尔通体感染的血清学诊断主要依靠显微免疫荧光测定法（IFA），但是这种方法费时且标准化程度很低。该研究的目的是评估使用新型全自动VirClia®化学发光免疫分析法检测IgG和IgM抗汉森芽孢杆菌抗体的能力。包括81例明确的汉逊氏杆菌感染患者和80例其他疾病患者。所述VirClia®IgG抗体测定显示79.0％的灵敏度和93.8％用于诊断特异性B.通体感染。对于VirClia®IgM分析，结果之间的矛盾更大，灵敏度为42.0％，预测IFA IgM结果的特异性为98.2％。在另外11位因自身免疫抗体导致IFA无法解释的患者中，VirClia®检测能够提供有价值的定量结果。VirClia®IgG测定法显示出良好的分析和临床表现，可轻松整合到亨氏芽孢杆菌感染的诊断工作流程中。