The purpose of the work is to evaluate the effect of extracellular polymeric substance (EPS) on the surface properties of Streptococcus mutans, and on the interaction between the bacteria and anti-adhesion nanostructured surfaces. Interactions between Streptococcus mutans with or without EPS and nanostructured surfaces were measured with atomic force microscope. Acid-base, Van der Waals, and electrostatic interactions were evaluated according to XDLVO theory. The absorption of glucan was evaluated by quartz crystal microbalance method. Results indicate Streptococcus mutans adhere stronger on surfaces with lower surface energy in both EPS and non-EPS conditions, because acid-base interaction is dominant. It indicates the superhydrophobic surface is lack of durability and reliability in the underwater anti-adhesion. Larger adhesion forces of Streptococcus mutans are shown in EPS than non-EPS conditions. That is due to more hydrogen bonds formed between glucan and substrate surfaces, and the EPS reducing surface energy and affecting acid-base interaction.

这项工作的目的是评估细胞外聚合物（EPS）对变形链球菌的表面性质以及细菌与抗粘附纳米结构表面之间相互作用的影响。用原子力显微镜测量变异链球菌在有无EPS和纳米结构表面之间的相互作用。根据XDLVO理论评估了酸碱，范德华力和静电相互作用。通过石英晶体微量天平法评价葡聚糖的吸收。结果表明变形链球菌在EPS和非EPS条件下，它们在具有较低表面能的表面上具有更强的附着力，因为酸碱相互作用占主导地位。这表明超疏水表面在水下抗粘连中缺乏耐久性和可靠性。与非EPS条件相比，EPS中显示出变形链球菌的粘附力更大。这是由于葡聚糖和底物表面之间形成了更多的氢键，EPS降低了表面能并影响酸碱相互作用。