The secretion disorder of sex hormones is the source that leads to the occurrence of many diseases such as polycystic ovarian syndrome (PCOS), hyperandrogenism and so on. There exist physiological changes in human body when slight fluctuations in concentrations of sex hormones happen. Therefore, it's of great significance for accurate detection of sex hormones in human body. In this work, TpPa-1 COF solid-phase microextraction (SPME) fiber was prepared using high-efficient in-situ synthesis strategy and coupled with HPLC-MS/MS to detect three sex hormones, including Progesterone (P), testosterone (T) and dehydroepiandrosterone (DHEA) in human serum. The thickness of the coating reached 7 μm within 2 h. Under the optimal conditions, the established method presented low limit of detections (LODs, ≤ 0.75 ng/mL), wide linear ranges (0.100-100 ng mL-1) and good reproducibility, and three sex hormones (T, P, DHEA) were successfully detected and quantified in human serum. In conclusion, the established SPME method presented high-efficient fiber preparation and good analytical performances of sex hormone detection, therefore was in great potential for application in clinical.

中文翻译：

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用于检测血清中性激素的原位逐层合成 TpPa-1 COF 固相微萃取纤维

性激素分泌紊乱是导致多囊卵巢综合征（PCOS）、高雄激素血症等多种疾病发生的根源。当性激素浓度发生轻微波动时，人体就会发生生理变化。因此，对准确检测人体内的性激素具有重要意义。在这项工作中，采用高效原位合成策略制备 TpPa-1 COF 固相微萃取 (SPME) 纤维，并结合 HPLC-MS/MS 检测三种性激素，包括孕酮 (P)、睾酮 (T ) 和人血清中的脱氢表雄酮 (DHEA)。涂层厚度在 2 小时内达到 7 μm。在最佳条件下，所建立的方法检测限低（LODs，≤ 0.75 ng/mL），线性范围宽（0. 100-100 ng mL-1) 和良好的重现性，在人血清中成功检测和定量了三种性激素（T、P、DHEA）。综上所述，所建立的SPME方法具有高效的纤维制备和良好的性激素检测分析性能，具有很大的临床应用潜力。