The razor clam, Sinonovacula constricta, is an important economic marine shellfish, and its larval development involves obvious morphological and physiological changes. MicroRNA plays a key role in the physiological changes of the organism through regulating targeted mRNA. This study performed miRNA–mRNA sequencing for eight different developmental stages of S. constricta using Illumina sequencing. A total of 2156 miRNAs were obtained, including 2069 known miRNAs and 87 novel miRNAs. In addition, target genes were predicted for key miRNAs differentially expressed between adjacent development samples by integrating the mRNA transcriptome. Further analysis revealed that the differentially expressed genes were enriched in complement activation, alternative pathways, translation, and negative regulation of monocyte molecular protein-1 production. KEGG pathway annotation showed significant enrichment in the regulation of the ribosome, phagosome, tuberculosis and fluid shear stress, and atherosclerosis. Ten mRNAs and ten miRNAs that are related to larval metamorphosis were identified using real-time PCR. Furthermore, the double luciferase experiment validated the negative regulatory relationship between miR-133 and peroxisome proliferator-activated receptor-γ (PPAR-γ). These results indicated that the target genes regulated by these differentially expressed miRNAs may play an important regulatory role in the metamorphosis development of S. constricta.

剃刀蛤（Sinonovacula constricta）是一种重要的经济海洋贝类，其幼体发育涉及明显的形态和生理变化。MicroRNA通过调节目标mRNA在生物的生理变化中起关键作用。这项研究对收缩链球菌的八个不同发育阶段进行了miRNA-mRNA测序。使用Illumina测序。共获得2156个miRNA，包括2069个已知miRNA和87个新miRNA。此外，通过整合mRNA转录组，可以预测相邻发育样品之间差异表达的关键miRNA的靶基因。进一步的分析表明，差异表达的基因在补体激活，替代途径，翻译和单核细胞分子蛋白质1产生的负调控中富集。KEGG通路注释显示在核糖体，吞噬体，结核和体液切应力以及动脉粥样硬化的调节中显着丰富。使用实时PCR鉴定了与幼虫变态相关的十个mRNA和十个miRNA。此外，双重荧光素酶实验验证了miR-133与过氧化物酶体增殖物激活的受体-γ（PPAR-γ）之间的负调控关系。这些结果表明，由这些差异表达的miRNA调控的靶基因可能在其变态发展中起重要的调控作用。收缩链球菌。