Avian leukosis virus (ALV) is associated with immune suppression, neoplasia, and reduced performance in chickens. In this study, two strains of ALV were isolated from Luxi gamecocks by DF-1 cell culture and identified by PCR, immunofluorescence assay, and sequencing of the viral genome. These strains were found to be novel recombinant viruses with nucleotide sequence identity of over 93.0% in the LTR and 94.4% in U3 to ALV-J, over 95.0% in the 5’UTR to ALV-C, over 93.4% in gp85 to ALV-B, and over 96.0% in gp37 to ALV-E. These results indicate that these two isolates are recombinants between ALV-J, ALV-C, ALV-E and ALV-B.

禽白血病病毒（ALV）与免疫抑制，肿瘤形成和鸡性能下降有关。在这项研究中，通过DF-1细胞培养从Lux西game鸡中分离出两株ALV菌株，并通过PCR，免疫荧光测定和病毒基因组测序进行鉴定。发现这些菌株是新型重组病毒，其核苷酸序列同一性在LTR中超过93.0％，在U3中对ALV-J达到94.4％，在5'UTR中对ALV-C超过95.0％，对gp85对ALV-9LV -B，以及gp37中超过96.0％的ALV-E。这些结果表明这两个分离株是ALV-J，ALV-C，ALV-E和ALV-B之间的重组体。