The substantial use of fungal enzymes to degrade lignocellulosic plant biomass has widely been attributed to the extensive requirement of powerful enzyme-producing fungal strains. In this study, a two-step screening procedure-a miniaturized culture method and shaking-flask fermentation-was proposed and demonstrated for finding cellulolytic fungi. Two hundred ninety-seven fungal strains were isolated from several cellulose-containing samples found in two different locations in Thailand. By using this screening strategy, 9 fungal strains were selected based on their potential for cellulase production. Based on sequence-based identification of these fungal isolates, 4 species in 4 genera were identified: Aspergillus terreus (3 strains: AG466, AG438 and AG499), Penicillium oxalicum (4 strains: AG452, AG496, AG498 and AG559), Talaromyces siamensis (1 strain: AG548) and Trichoderma afroharzianum (1 strain: AG500). After examining their lignocellulose degradation capacity, our data showed that P. oxalicum AG452 exhibited the highest glucose yield after saccharification of pretreated sugarcane trash, cassava pulp and coffee silver skin. In addition, Ta. siamensis AG548 produced the highest glucose yield after hydrolysis of pretreated sugarcane bagasse. Our study demonstrated that the proposed two-step screening strategy can be further applied for discovering cellulolytic fungal potentials isolated from various environmental samples. The fungal strains isolated in this study can be further applied for bioconversion of agricultural lignocellulosic residues into valuable biotechnological products.

中文翻译：

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对纤维素分解真菌及其农业木质纤维素降解的小型化培养筛选。

大量使用真菌酶来降解木质纤维素植物生物质已广泛归因于对强大的产酶真菌菌株的广泛需求。在这项研究中，提出并证明了一种两步筛选程序——小型化培养法和摇瓶发酵法——用于寻找纤维素分解真菌。从在泰国两个不同地点发现的几个含纤维素样品中分离出 297 株真菌菌株。通过使用这种筛选策略，根据它们的纤维素酶生产潜力选择了 9 种真菌菌株。基于对这些真菌分离株的基于序列的鉴定，鉴定出 4 个属的 4 个种：土曲霉（3 个菌株：AG466、AG438 和 AG499）、草酸青霉菌（4 个菌株：AG452、AG496、AG498 和 AG559）、Talaromyces siamensis（1 个菌株：AG548）和Trichoderma afroharzianum（1 个菌株：AG500）。在检查了它们的木质纤维素降解能力后，我们的数据表明，P. oxalicum AG452 在预处理甘蔗垃圾、木薯果肉和咖啡银皮糖化后表现出最高的葡萄糖产量。此外，塔。暹罗AG548 在预处理甘蔗渣水解后产生了最高的葡萄糖产量。我们的研究表明，所提出的两步筛选策略可以进一步应用于发现从各种环境样品中分离的纤维素分解真菌潜力。本研究中分离的真菌菌株可进一步应用于将农业木质纤维素残留物生物转化为有价值的生物技术产品。